Rabbit anti-Human Vang-like protein 1, VANGL1 Polyclonal Antibody | anti-VANGL1 antibody

Anti-Vang-like protein 1, VANGL1 antibody

Cat. Num. AAA355202

• Gene Names: VANGL1; LPP2; STB2; STBM2; KITENIN
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: Vang-like protein 1; VANGL1; Polyclonal Antibody; Anti-Vang-like protein 1; VANGL1 antibody; Polyclonal Anti- Vang-like protein 1; anti-VANGL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-VANGL1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:500); ICC (1:100-200)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of human VANGL1.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB)

Related Product Information for anti-VANGL1 antibody

Vang-like protein 1 (VANGL1), also called Loop-tail protein 2 homolog (LPP2), is encoded by the VANGL1 gene, This gene contains 8 exons[1] and it maps to chromosome 1p13[2]. The VANGL1 protein is a 524-amino acid, 65KD protein, which has 4 transmembrane domains and a C-terminal ser/thr-x-val motif. Yagyu et al. (2002) found that downregulation of VANGL1 expression suppressed growth of a human hepatoma cell line.[1] Kalabis et al. (2006) found that VANGL1 overexpression enhanced this migration and wound closure in cultured cells.[3]

References

1. Yagyu, R., Hamamoto, R., Furukawa, Y., Okabe, H., Yamamura, T., Nakamura, Y. Isolation and characterization of a novel human gene, VANGL1, as a therapeutic target for hepatocellular carcinoma. Int. J. Oncol. 20: 1173-1178, 2002. 2. Katoh, M. Molecular cloning and characterization of Strabismus 2 (STB2). Int. J. Oncol. 20: 993-998, 2002. 3. Kalabis, J., Rosenberg, I., Podolsky, D. K. Vangl1 protein acts as a downstream effector of intestinal trefoil factor (ITF)/TFF3 signaling and regulates wound healing of intestinal epithelium. J. Biol. Chem. 281: 6434-6441, 2006

NCBI and Uniprot Product Information

• NCBI GI #: 20373171
• NCBI GeneID: 81839
• NCBI Accession #: NP_620409.1
• NCBI GenBank Nucleotide #: NM_138959.2
• UniProt Accession #: Q8TAA9; Q5T1D3; Q5T1D4; Q86WG8; Q8N559
• Molecular Weight: 59,975 Da
• NCBI Official Full Name: vang-like protein 1 isoform 1
• NCBI Official Synonym Full Names: VANGL planar cell polarity protein 1
• NCBI Official Symbol: VANGL1
• NCBI Official Synonym Symbols: LPP2; STB2; STBM2; KITENIN
• NCBI Protein Information: vang-like protein 1; strabismus 2; van Gogh-like protein 1; loop-tail protein 2 homolog; vang-like 1 (van gogh, Drosophila); KAI1 C-terminal interacting tetraspanin
• UniProt Protein Name: Vang-like protein 1
• Protein Family: Vang-like protein
• UniProt Gene Name: VANGL1
• UniProt Synonym Gene Names: STB2; LPP2
• UniProt Entry Name: VANG1_HUMAN

NCBI Description

This gene encodes a member of the tretraspanin family. The encoded protein may be involved in mediating intestinal trefoil factor induced wound healing in the intestinal mucosa. Mutations in this gene are associated with neural tube defects. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

Subunit structure: Interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. The PDZ domain of DVL1, DVL2 and DVL3 is required for the interaction

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: According to Ref.1, ubiquitously expressed. According to Ref.2, expressed specifically in testis and ovary. Ref.1 Ref.2

Involvement in disease: Neural tube defects (NTD) [MIM:182940]: Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12Sacral defect with anterior meningocele (SDAM) [MIM:600145]: Form of caudal dysgenesis. It is present at birth and becomes symptomatic later in life, usually because of obstructive labor in females, chronic constipation, or meningitis. Inheritance is autosomal dominant.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11

Sequence similarities: Belongs to the Vang family.

Sequence caution: The sequence AAH32773.1 differs from that shown. Reason: Erroneous initiation.