Rabbit anti-Human, Mouse UVRAG Polyclonal Antibody | anti-UVRAG antibody

UVRAG, ID (UV Radiation Resistance-associated Gene Protein, p63, DHTX, VPS38) (Biotin)

Cat. Num. AAA6362149

• Gene Names: UVRAG; p63; DHTX; VPS38
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: UVRAG; Polyclonal Antibody; ID (UV Radiation Resistance-associated Gene Protein; p63; DHTX; VPS38) (Biotin); UV Radiation Resistance Associated Gene Protein; anti-UVRAG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-UVRAG antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375~404 amino acids from the center of human UVRAG.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-hUVRAG (Center) Pab (RB10847) in mouse heart tissue lysates. hUVRAG (Center)(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-UVRAG antibody

UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

Product Categories/Family for anti-UVRAG antibody

Antibodies; Cancer Markers

References

Liang, C., et al. Nat. Cell Biol. 8 (7), 688-699 (2006). Ionov, Y., et al. Oncogene 23 (3), 639-645 (2004). Goi, T., et al. Surg. Today 33 (9), 702-706 (2003). Iida, A., et al. Hum. Genet. 106 (3), 277-287 (2000). Perelman, B., et al. Genomics 41 (3), 397-405 (1997). Bekri, S., et al. Cytogenet. Cell Genet. 79 (1-2), 125-131 (1997). Teitz, T., et al. Gene 87 (2), 295-298 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 21687212
• NCBI GeneID: 7405
• NCBI Accession #: NP_003360
• NCBI GenBank Nucleotide #: NM_003369.3
• UniProt Accession #: Q9P2Y5; O00392; B3KTC1
• Molecular Weight: Predicted: 77 kDa; ; Observed: 75 kDa
• NCBI Official Full Name: UV radiation resistance-associated gene protein
• NCBI Official Synonym Full Names: UV radiation resistance associated
• NCBI Official Symbol: UVRAG
• NCBI Official Synonym Symbols: p63; DHTX; VPS38
• NCBI Protein Information: UV radiation resistance-associated gene protein; disrupted in heterotaxy; beclin 1 binding protein
• UniProt Protein Name: UV radiation resistance-associated gene protein
• Protein Family: UV radiation resistance associated protein
• UniProt Gene Name: UVRAG
• UniProt Entry Name: UVRAG_HUMAN

NCBI Description

This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

UVRAG: Forms a complex with BECN1, PIK3C3 and PIK3R4. A subpopulation of these complexes also harbor KIAA0226/Rubicon. UVRAG-containing complexes never including ATG14, both forming mutually exclusive complexes with BECN1 through direct competition. Highly expressed in brain, lung, kidney and liver.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: protein complex; lysosome; early endosome; late endosome; cytoplasm; phagocytic vesicle

Molecular Function: protein binding; SNARE binding; SH3 domain binding

Biological Process: entry of virus into host cell; DNA repair; positive regulation of autophagy