Rabbit USP8 Polyclonal Antibody | anti-USP8 antibody

Anti-USP8 Antibody

Cat. Num. AAA416915

• Gene Names: USP8; UBPY; SPG59; HumORF8
• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: USP8; Polyclonal Antibody; Anti-USP8 Antibody; anti-USP8 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 4083

• Applicable Applications for anti-USP8 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Immunogen: Rabbit polyclonal USP8(1) antibody was raised against a recombinate human USP8 protein 886-1096aa (BC110590).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-USP8 antibody

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

References

(1) Xia,R., Jia,H., Fan,J., Liu,Y. and Jia,J. USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. PLoS Biol. 10 (1), E1001238 (2012)

NCBI and Uniprot Product Information

• NCBI GI #: 115527450
• NCBI GeneID: 9101
• Molecular Weight: 115,080 Da
• NCBI Official Full Name: Homo sapiens ubiquitin specific peptidase 8, mRNA
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: USP8
• NCBI Official Synonym Symbols: UBPY; SPG59; HumORF8
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8; deubiquitinating enzyme 8; ubiquitin isopeptidase Y; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: USP8
• UniProt Entry Name: UBP8_HUMAN

NCBI Description

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; extrinsic to plasma membrane; Golgi apparatus; early endosome; cytoplasm; acrosome; midbody; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SH3 domain binding

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; endosome organization and biogenesis; cytokinesis after mitosis; Ras protein signal transduction