Western Blot (WB)
(Western blot analysis USP49 using Jurkat whole cell lysates)
Rabbit USP49 Polyclonal Antibody | anti-USP49 antibody
USP49 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
USP49; Polyclonal Antibody; USP49 Antibody; Deubiquitinating enzyme 49; MGC20741; Ubiquitin carboxyl-terminal hydrolase 49; ubiquitin specific peptidase 49; Ubiquitin thiolesterase 49; Ubiquitin-specific-processing protease 49; UBP49; UBP49_HUMAN; USP49 ubiquitin specific peptidase 49; anti-USP49 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
USP49 antibody detects endogenous levels of total USP49
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
688
Applicable Applications for anti-USP49 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human USP49
Subcellular Location
Nucleus
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis USP49 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis USP49 using Jurkat whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610307 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610307 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-USP49 antibody
Function: Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.
Subunit Structure: Component of a complex with RUVBL1 and PSMC5.
Similarity: Belongs to the peptidase C19 family.
Subunit Structure: Component of a complex with RUVBL1 and PSMC5.
Similarity: Belongs to the peptidase C19 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 79 kDa
Predicted: 80 kDa
Predicted: 80 kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 49 isoform a
NCBI Official Synonym Full Names
ubiquitin specific peptidase 49
NCBI Official Symbol
USP49
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 49
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 49
Protein Family
UniProt Gene Name
USP49
Uniprot Description
Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.
Research Articles on USP49
Similar Products
Product Notes
The USP49 usp49 (Catalog #AAA9610307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP49 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP49 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the USP49 usp49 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP49, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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