Rabbit USP32 Polyclonal Antibody | anti-USP32 antibody

USP32 polyclonal antibody

Cat. Num. AAA3014932

• Gene Names: USP32; USP10; NY-REN-60
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: USP32; Polyclonal Antibody; USP32 polyclonal antibody; Ubiquitin carboxyl-terminal hydrolase 32; Deubiquitinating enzyme 32; Renal carcinoma antigen NY-REN-60; Ubiquitin thioesterase 32; Ubiquitin-specific-processing protease 32; USP10; anti-USP32 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: USP32 polyclonal antibody detects endogenous levels of USP32 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-USP32 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human USP32
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of USP32 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: PC12 whole cell lysateLaneD: AML-12 whole cell lysate)

Related Product Information for anti-USP32 antibody

Ubiquitin-specific protease (USP6) is a 1,406 amino acid protein that is very hydrophilic and has 2 charge clusters that are characteristic of nucleic acidbinding regions. The USP6 gene is oncogenic and originated from the chimeric fusion of two genes: USP32 and TBC1D3. USP32 is an ancient, highly conserved gene, whereas the TBC1D3 gene came from a relatively recent segmental duplication. This duplication is absent in most other mammals and shows rapid amplification and propagation throughout the primate lineage. The chimeric USP6, however, only exists in the hominoid lineage of primates, so it may have contributed to hominoid speciation. USP6 is testis-specific, suggesting an implication in the emergence of reproductive barriers.

NCBI and Uniprot Product Information

• NCBI GI #: 22550104
• NCBI GeneID: 84669
• NCBI Accession #: NP_115971.2
• NCBI GenBank Nucleotide #: NM_032582.3
• UniProt Accession #: Q8NFA0; Q9BX85; Q9Y591
• Molecular Weight: 181,656 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 32
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 32
• NCBI Official Symbol: USP32
• NCBI Official Synonym Symbols: USP10; NY-REN-60
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 32; ubiquitin thioesterase 32; deubiquitinating enzyme 32; ubiquitin thiolesterase 32; ubiquitin specific protease 32; renal carcinoma antigen NY-REN-60; ubiquitin-specific-processing protease 32
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 32
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP32
• UniProt Synonym Gene Names: USP10
• UniProt Entry Name: UBP32_HUMAN

Uniprot Description

USP32: Belongs to the peptidase C19 family.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: Golgi apparatus; membrane; cytoplasm

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; calcium ion binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination