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Western Blot (WB) (Western blot (WB) analysis of USP32 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: PC12 whole cell lysateLaneD: AML-12 whole cell lysate)

Rabbit USP32 Polyclonal Antibody | anti-USP32 antibody

USP32 polyclonal antibody

Gene Names
USP32; USP10; NY-REN-60
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
USP32; Polyclonal Antibody; USP32 polyclonal antibody; Ubiquitin carboxyl-terminal hydrolase 32; Deubiquitinating enzyme 32; Renal carcinoma antigen NY-REN-60; Ubiquitin thioesterase 32; Ubiquitin-specific-processing protease 32; USP10; anti-USP32 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
USP32 polyclonal antibody detects endogenous levels of USP32 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-USP32 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human USP32
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of USP32 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: PC12 whole cell lysateLaneD: AML-12 whole cell lysate)

Western Blot (WB) (Western blot (WB) analysis of USP32 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: MCF-7 whole cell lysateLaneC: PC12 whole cell lysateLaneD: AML-12 whole cell lysate)
Related Product Information for anti-USP32 antibody
Ubiquitin-specific protease (USP6) is a 1,406 amino acid protein that is very hydrophilic and has 2 charge clusters that are characteristic of nucleic acidbinding regions. The USP6 gene is oncogenic and originated from the chimeric fusion of two genes: USP32 and TBC1D3. USP32 is an ancient, highly conserved gene, whereas the TBC1D3 gene came from a relatively recent segmental duplication. This duplication is absent in most other mammals and shows rapid amplification and propagation throughout the primate lineage. The chimeric USP6, however, only exists in the hominoid lineage of primates, so it may have contributed to hominoid speciation. USP6 is testis-specific, suggesting an implication in the emergence of reproductive barriers.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
181,656 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 32
NCBI Official Synonym Full Names
ubiquitin specific peptidase 32
NCBI Official Symbol
USP32
NCBI Official Synonym Symbols
USP10; NY-REN-60
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 32; ubiquitin thioesterase 32; deubiquitinating enzyme 32; ubiquitin thiolesterase 32; ubiquitin specific protease 32; renal carcinoma antigen NY-REN-60; ubiquitin-specific-processing protease 32
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 32
UniProt Gene Name
USP32
UniProt Synonym Gene Names
USP10
UniProt Entry Name
UBP32_HUMAN

Uniprot Description

USP32: Belongs to the peptidase C19 family.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: Golgi apparatus; membrane; cytoplasm

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; calcium ion binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination

Research Articles on USP32

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Product Notes

The USP32 usp32 (Catalog #AAA3014932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP32 polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the USP32 usp32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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