Rabbit USP30 Polyclonal Antibody | anti-USP30 antibody

USP30 polyclonal antibody

Cat. Num. AAA3000109

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: USP30; Polyclonal Antibody; USP30 polyclonal antibody; Ubiquitin carboxyl-terminal hydrolase 30; Deubiquitinating enzyme 30; Ubiquitin thioesterase 30; Ubiquitin-specific-processing protease 30; Ub-specific protease 30; anti-USP30 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: USP30 polyclonal antibody detects endogenous levels of USP30 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Applicable Applications for anti-USP30 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human USP30.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of USP30 polyclonal antibody at 1:500 dilutionLane1:Aml-12 whole cell lysate(40ug)Lane2:H9C2 whole cell lysate(40ug)Lane3:HEK293T whole cell lysate(20ug)Lane4:The muscle tissue lysate of mouse(40ug)Lane5:Panc1 whole cell lysate(20ug)Lane6:HepG2 whole cell lysate(20ug))

Related Product Information for anti-USP30 antibody

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP30 (ubiquitin specific peptidase 30) is a 517 amino acid protein that localizes to the mitochondrion and belongs to the ubiquitin-specific protease family.Expressed in pancreas, kidney, liver and skeletal muscle, USP30 functions to catalyze the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP30 maps to human chromosome 12q24.11, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 164607167
• NCBI GeneID: 84749
• NCBI Accession #: NP_116052.2
• NCBI GenBank Nucleotide #: NM_032663.3
• UniProt Accession #: Q70CQ3; Q8WTU7; Q96JX4; Q9BSS3
• Molecular Weight: 58,503 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 30
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 30
• NCBI Official Symbol: USP30
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 30; ub-specific protease 30; ubiquitin thioesterase 30; deubiquitinating enzyme 30; ubiquitin thiolesterase 30; ubiquitin specific protease 30; ubiquitin-specific protease 30; ubiquitin-specific-processing protease 30
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 30
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP30
• UniProt Synonym Gene Names: Ub-specific protease 30
• UniProt Entry Name: UBP30_HUMAN

NCBI Description

USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).[supplied by OMIM, Dec 2008]

Uniprot Description

USP30: May participate in the maintenance of mitochondrial morphology. Belongs to the peptidase C19 family.

Protein type: Membrane protein, integral; EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; mitochondrial fusion; mitochondrion degradation