Goat USP28 Polyclonal Antibody | anti-USP28 antibody

Goat Polyclonal to Human USP28

Cat. Num. AAA243709

• Reactivity: Gibbon, Bovine, Dog, Horse, Human, Monkey, Pig, Rabbit; Predicted Reactivity: Bat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: USP28; Polyclonal Antibody; Goat Polyclonal to Human USP28; Anti-USP28 Antibody (Internal) IHC-plus; Deubiquitinating enzyme 28; KIAA1515; Ubiquitin specific protease 28; Ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Human USP28; anti-USP28 antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Dog, Horse, Human, Monkey, Pig, Rabbit; Predicted Reactivity: Bat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human USP28.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1077

• Applicable Applications for anti-USP28 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (2.5 - 3.75 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SVELKHYIQEDN from an internal region of human USP28 (NP_065937.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum (100%); Elephant, Bat (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: USP28 antibody was raised against synthetic peptide C-SVELKHYIQEDN from an internal region of human USP28 (NP_065937.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum (100%); Elephant, Bat (92%); Mouse, Rat, Hamster (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-USP28 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-USP28 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Product Categories/Family for anti-USP28 antibody

Family: Protease; Subfamily: Cysteine C19

NCBI and Uniprot Product Information

• NCBI GI #: 16507200
• NCBI GeneID: 57646
• NCBI Accession #: NP_065937.1
• NCBI GenBank Nucleotide #: NM_020886.3
• UniProt Accession #: Q96RU2; Q6NZX9; Q9P213; B0YJC0; B0YJC1
• Molecular Weight: 66,243 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 28 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 28
• NCBI Official Symbol: USP28
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 28; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin specific protease 28; ubiquitin-specific-processing protease 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 28
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP28
• UniProt Synonym Gene Names: KIAA1515
• UniProt Entry Name: UBP28_HUMAN

NCBI Description

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).[supplied by OMIM, Mar 2008]

Uniprot Description

USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA damage checkpoint; response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis