Rabbit anti-Human, Mouse USP25 Polyclonal Antibody | anti-USP25 antibody

USP25 Antibody

Cat. Num. AAA9629837

• Gene Names: USP25; USP21
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: USP25; Polyclonal Antibody; USP25 Antibody; Deubiquitinating enzyme 25; Ubiquitin carboxyl-terminal hydrolase 25; Ubiquitin specific peptidase 25; ubiquitin specific protease 25; ubiquitin specific protease USP25; Ubiquitin thiolesterase 25; Ubiquitin-specific-processing protease 25; UBP25_HUMAN; USP on chromosome 21; USP21; anti-USP25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: USP25 Antibody detects endogenous levels of USP25.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-USP25 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human USP25.
• Conjugation: Unconjugated
• Similarity: Belongs to the peptidase C19 family.
• Post Translational Modifications: Acetylated.Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
• Subunit Structure: Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.
• Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
• Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
• Note: ome transient punctuate nuclear location in myotubes during myocyte development.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-USP25 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 302393833
• NCBI GeneID: 29761
• UniProt Accession #: Q9UHP3; Q6DHZ9; Q9H9W1; C0LSZ0
• Molecular Weight: 1055
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 25
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 25
• NCBI Official Symbol: USP25
• NCBI Official Synonym Symbols: USP21
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 25; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 25
• UniProt Gene Name: USP25
• UniProt Synonym Gene Names: USP21
• UniProt Entry Name: UBP25_HUMAN

NCBI Description

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

Uniprot Description

USP25: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: peptidase activity; protein binding; SUMO binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein modification process; proteolysis