Rabbit anti-Human USP14 Polyclonal Antibody | anti-USP14 antibody

USP14, CT (USP14, TGT, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14) (AP)

Cat. Num. AAA6361839

• Gene Names: USP14; TGT
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP14; Polyclonal Antibody; CT (USP14; TGT; Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14) (AP); Ubiquitin-specific-processing protease 14; anti-USP14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-USP14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: USP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-383 amino acids from the C-terminal region of human USP14.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(USP14 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the USP14 antibody detected the USP14 protein (arrow).)

Related Product Information for anti-USP14 antibody

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-USP14 antibody

Antibodies; Ubiquitin

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Chen, P.C., et al. J. Neurosci. 29(35):10909-10919(2009). Mines, M.A., et al. J. Biol. Chem. 284(9):5742-5752(2009). Nagai, A., et al. Biochem. Biophys. Res. Commun. 379(4):995-1000(2009). Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4827050
• NCBI GeneID: 9097
• NCBI Accession #: NP_005142.1
• NCBI GenBank Nucleotide #: NM_005151.3
• UniProt Accession #: P54578; Q53XY5
• Molecular Weight: 56,069 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 14 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• NCBI Official Symbol: USP14
• NCBI Official Synonym Symbols: TGT
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 14; ubiquitin thioesterase 14; deubiquitinating enzyme 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; ubiquitin-specific-processing protease 14; tRNA-guanine transglycosylase, 60-kD subunit
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 14
• UniProt Gene Name: USP14
• UniProt Synonym Gene Names: TGT
• UniProt Entry Name: UBP14_HUMAN

Uniprot Description

USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Protein type: Protease; Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: proteasome complex; cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; synapse; nucleus

Molecular Function: protein binding; tRNA guanylyltransferase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; endopeptidase inhibitor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; synaptic transmission; protein deubiquitination; regulation of chemotaxis