Rabbit USP13 Polyclonal Antibody | anti-USP13 antibody

USP13 Polyclonal Antibody

Cat. Num. AAA3009575

• Gene Names: USP13; ISOT3; IsoT-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: USP13; Polyclonal Antibody; USP13 Polyclonal Antibody; ISOT3; IsoT-3; anti-USP13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP13 polyclonal antibody detects endogenous levels of USP13 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 863

• Applicable Applications for anti-USP13 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human USP13
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using USP13 antibody.)

Related Product Information for anti-USP13 antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and 2 UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.

NCBI and Uniprot Product Information

• NCBI GI #: 215598688
• NCBI GeneID: 8975
• NCBI Accession #: NP_003931.2
• NCBI GenBank Nucleotide #: NP_003931.2
• UniProt Accession #: Q92995
• Molecular Weight: ~ 97kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 13
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 13
• NCBI Official Symbol: USP13
• NCBI Official Synonym Symbols: ISOT3; IsoT-3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 13
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 13
• UniProt Gene Name: USP13
• UniProt Synonym Gene Names: ISOT3; ISOT-3
• UniProt Entry Name: UBP13_HUMAN

Uniprot Description

USP13: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q26.2-q26.3

Cellular Component: lysosome

Molecular Function: omega peptidase activity; ubiquitin binding; protein binding; zinc ion binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; protein stabilization; melanocyte differentiation; autophagy; regulation of autophagy