Rabbit anti-Human, Mouse USF1 Polyclonal Antibody | anti-USF1 antibody

USF1, ID (USF1, BHLHB11, USF, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1) (PE)

Cat. Num. AAA6361783

• Gene Names: USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USF1; Polyclonal Antibody; ID (USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1) (PE); Major late transcription factor 1; anti-USF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-USF1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).)

Flow Cytometry (FC/FACS)

(USF1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly. )

Immunohistochemistry (IHC)

(USF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-USF1 antibody

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-USF1 antibody

Antibodies; Transcription Factors

References

Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Bussiere, F.I., et al. Cell. Microbiol. 12(8):1124-1133(2010). Naukkarinen, J., et al. Circ Cardiovasc Genet 2(5):522-529(2009). Singmann, P., et al. Obes Facts 2(4):235-242(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6005934
• NCBI GeneID: 7391
• NCBI Accession #: NP_009053.1
• NCBI GenBank Nucleotide #: NM_007122.4
• UniProt Accession #: P22415; Q5SY46; Q7Z5Y1; B2RBZ4
• Molecular Weight: 27,372 Da
• NCBI Official Full Name: upstream stimulatory factor 1 isoform 1
• NCBI Official Synonym Full Names: upstream transcription factor 1
• NCBI Official Symbol: USF1
• NCBI Official Synonym Symbols: UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• NCBI Protein Information: upstream stimulatory factor 1; major late transcription factor 1; class B basic helix-loop-helix protein 11
• UniProt Protein Name: Upstream stimulatory factor 1
• Protein Family: Upstream stimulatory factor
• UniProt Gene Name: USF1
• UniProt Synonym Gene Names: BHLHB11; USF; bHLHb11
• UniProt Entry Name: USF1_HUMAN

Uniprot Description

USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; lipid homeostasis; glucose metabolic process; late viral mRNA transcription; glucose homeostasis; regulation of transcription from RNA polymerase II promoter; negative regulation of fibrinolysis; cellular response to insulin stimulus; regulation of transcription from RNA polymerase II promoter by glucose; response to hypoxia; regulation of transcription by carbon catabolites; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; response to UV

Disease: Hyperlipidemia, Combined, 1