Goat anti-Human UROD Polyclonal Antibody | anti-UROD antibody

Goat Polyclonal to Human UROD

Cat. Num. AAA248386

• Gene Names: UROD; PCT; UPD
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: UROD; Polyclonal Antibody; Goat Polyclonal to Human UROD; Anti-UROD Antibody (Internal) IHC-plus; URO-D; Uroporphyrinogen decarboxylase; PCT; UPD; Human UROD; anti-UROD antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human UROD
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 367

• Applicable Applications for anti-UROD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kD band observed in lysates of cell line K56 (calculated MW of 40.8kD according to NP_000365.3). Recommended concentration: 0.1-0.3 ug/ml. An additional band of unknown...
• Target Species: Human
• Immunogen Description: Synthetic peptide KARECVGKTVTLQGN from an internal region of human UROD (NP_000365.3). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Bovine, Cat, Horse, Rabbit, Guinea pig (93%); Galago, Mouse, Dog, Pig, Opossum (87%).
• Immunogen Type: Synthetic peptide
• Immunogen: UROD antibody was raised against synthetic peptide KARECVGKTVTLQGN from an internal region of human UROD (NP_000365.3). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Bovine, Cat, Horse, Rabbit, Guinea pig (93%); Galago, Mouse, Dog, Pig, Opossum (87%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-UROD antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(UROD antibody (0.1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 71051616
• NCBI GeneID: 7389
• NCBI Accession #: NP_000365.3
• NCBI GenBank Nucleotide #: NM_000374.4
• UniProt Accession #: P06132; Q16863; Q16883; Q53YB8; Q53ZP6; Q6IB28; Q9BUZ0; A8K762
• Molecular Weight: 40,787 Da
• NCBI Official Full Name: uroporphyrinogen decarboxylase
• NCBI Official Synonym Full Names: uroporphyrinogen decarboxylase
• NCBI Official Symbol: UROD
• NCBI Official Synonym Symbols: PCT; UPD
• NCBI Protein Information: uroporphyrinogen decarboxylase; uroporphyrinogen III decarboxylase
• UniProt Protein Name: Uroporphyrinogen decarboxylase
• UniProt Gene Name: UROD
• UniProt Synonym Gene Names: UPD; URO-D
• UniProt Entry Name: DCUP_HUMAN

NCBI Description

This gene encodes an enzyme in the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are known to cause familial porphyria cutanea tarda and hepatoerythropoetic porphyria.[provided by RefSeq, Aug 2010]

Uniprot Description

UROD: Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III. Defects in UROD are the cause of familial porphyria cutanea tarda (FPCT); also known as porphyria cutanea tarda type II. FPCT is an autosomal dominant disorder characterized by light-sensitive dermatitis, with onset in later life. It is associated with the excretion of large amounts of uroporphyrin in the urine. Iron overload is often present in association with varying degrees of liver damage. Besides the familial form of PCT, a relatively common idiosyncratic form is known in which only the liver enzyme is reduced. This form is referred to as porphyria cutanea tarda sporadic type or type I. PCT type I occurs sporadically as an unusual accompaniment of common hepatic disorders such as alcohol- associated liver disease. Defects in UROD are the cause of hepatoerythropoietic porphyria (HEP). HEP is a rare autosomal recessive disorder. It is the severe form of cutaneous porphyria, and presents in infancy. The level of UROD is very low in erythrocytes and cultured skin fibroblasts, suggesting that HEP is the homozygous state for porphyria cutanea tarda. Belongs to the uroporphyrinogen decarboxylase family.

Protein type: EC 4.1.1.37; Lyase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: uroporphyrinogen decarboxylase activity

Biological Process: porphyrin metabolic process; protoporphyrinogen IX biosynthetic process; heme biosynthetic process

Disease: Porphyria Cutanea Tarda