Rabbit anti-Human Uridine Monophosphate Kinase 2 Polyclonal Antibody | anti-UCK2 antibody

Uridine Monophosphate Kinase 2, NT (Cytidine monophosphokinase 2, Testis-specific protein, UCK 2, UK, UMPK, Uridine-cytidine kinase 2, Uridine monophosphokinase 2) (PE)

Cat. Num. AAA6504891

• Gene Names: UCK2; UK; UMPK; TSA903
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Uridine Monophosphate Kinase 2; Polyclonal Antibody; NT (Cytidine monophosphokinase 2; Testis-specific protein; UCK 2; UK; UMPK; Uridine-cytidine kinase 2; Uridine monophosphokinase 2) (PE); anti-UCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UCK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-UCK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:250; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human UCK2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-UCK2 Antibody (N-term) pre-incubated with and without blocking peptide (BP7192a) in Hela cell line lysate. UCK2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-UCK2 antibody

Antibodies; Protein Kinases

References

1. Murata, D., et al., Drug Metab. Dispos. 32(10):1178-1182 (2004). 2. Pasti, C., et al., Eur. J. Biochem. 270(8):1784-1790 (2003). 3. Liou, J.Y., et al., Cancer Res. 62(6):1624-1631 (2002). 4. Pearman, A.T., et al., Life Sci. 69(20):2361-2370 (2001). 5. Van Rompay, A.R., et al., Mol. Pharmacol. 56(3):562-569 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 18699734
• NCBI GeneID: 7371
• NCBI Accession #: NP_036606
• NCBI GenBank Nucleotide #: NM_012474.4
• UniProt Accession #: Q9BZX2; Q5VV91; Q7KZV3; Q92528; Q96KG5; Q9BU42
• Molecular Weight: 30.3kDa (269aa), confirmed by MALDI-TOF
• NCBI Official Full Name: uridine-cytidine kinase 2
• NCBI Official Synonym Full Names: uridine-cytidine kinase 2
• NCBI Official Symbol: UCK2
• NCBI Official Synonym Symbols: UK; UMPK; TSA903
• NCBI Protein Information: uridine-cytidine kinase 2
• UniProt Protein Name: Uridine-cytidine kinase 2
• Protein Family: Uridine-cytidine kinase
• UniProt Gene Name: UCK2
• UniProt Synonym Gene Names: UMPK; UCK 2
• UniProt Entry Name: UCK2_HUMAN

NCBI Description

This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010]

Uniprot Description

UCK2: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.48; Nucleotide Metabolism - pyrimidine; Kinase, other; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: intracellular membrane-bound organelle; cytosol

Molecular Function: nucleoside kinase activity; uridine kinase activity; ATP binding

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; CMP salvage; pyrimidine nucleoside salvage; feeding behavior; response to axon injury; phosphorylation