Western Blot (WB)
(Western Blot: Sample: Porcine Heart Tissue.)
Rabbit anti-Human Uracil DNA Glycosylase Polyclonal Antibody | anti-UNG antibody
Polyclonal Antibody to Uracil DNA Glycosylase (UNG)
Gene Names
UNG; DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Uracil DNA Glycosylase; Polyclonal Antibody; Polyclonal Antibody to Uracil DNA Glycosylase (UNG); anti-UNG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against UNG. It has beenselected for its ability to recognize UNG in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.37mg/ml (varies by lot)
Sequence Length
218
Applicable Applications for anti-UNG antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
UNG (Met1~Leu313)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Pig
Conjugate
No Conjugate
Immunogen
Recombinant UNG (Met1~Leu313) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2078801
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Porcine Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Porcine Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant UNG, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant UNG, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded skeletal muscle tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded skeletal muscle tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,924 Da
NCBI Official Full Name
uracil-DNA glycosylase isoform UNG1
NCBI Official Synonym Full Names
uracil DNA glycosylase
NCBI Official Symbol
UNG
NCBI Official Synonym Symbols
DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
NCBI Protein Information
uracil-DNA glycosylase
UniProt Protein Name
Uracil-DNA glycosylase
Protein Family
UniProt Gene Name
UNG
UniProt Synonym Gene Names
UDG
NCBI Description
This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]
Uniprot Description
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Research Articles on UNG
Similar Products
Product Notes
The UNG ung (Catalog #AAA2028461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Uracil DNA Glycosylase (UNG) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Uracil DNA Glycosylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the UNG ung for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Uracil DNA Glycosylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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