Rabbit Uncoupling Protein 1 Polyclonal Antibody | anti-UCP1 antibody

Uncoupling Protein 1 (UCP1, UCP, SLC25A7) (Biotin)

Cat. Num. AAA6442558

• Gene Names: UCP1; UCP; SLC25A7
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Uncoupling Protein 1; Polyclonal Antibody; Uncoupling Protein 1 (UCP1; UCP; SLC25A7) (Biotin); anti-UCP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Uncoupling Protein 1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-UCP1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human Uncoupling Protein 1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UCP1 antibody.)

Product Categories/Family for anti-UCP1 antibody

Antibodies; Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11225256
• NCBI GeneID: 7350
• NCBI Accession #: NP_068605
• NCBI GenBank Nucleotide #: NM_021833.4
• UniProt Accession #: P25874; Q13218; Q4KMZ3; Q68G66
• Molecular Weight: Predicted: 34 kDa; ; Observed: 36 kDa
• NCBI Official Full Name: mitochondrial brown fat uncoupling protein 1
• NCBI Official Synonym Full Names: uncoupling protein 1 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP1
• NCBI Official Synonym Symbols: UCP; SLC25A7
• NCBI Protein Information: mitochondrial brown fat uncoupling protein 1; thermogenin; solute carrier family 25 member 7
• UniProt Protein Name: Mitochondrial brown fat uncoupling protein 1
• Protein Family: Mitochondrial brown fat uncoupling protein
• UniProt Gene Name: UCP1
• UniProt Synonym Gene Names: SLC25A7; UCP; UCP 1
• UniProt Entry Name: UCP1_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]

Uniprot Description

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: oxidative phosphorylation uncoupler activity

Biological Process: regulation of transcription from RNA polymerase II promoter; cellular metabolic process; proton transport; brown fat cell differentiation; mitochondrial transport

Disease: Obesity