Rabbit anti-Human ULK2 Polyclonal Antibody | anti-ULK2 antibody

ULK2, ID (S323) (ULK2, KIAA0623, Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2) (FITC)

Cat. Num. AAA6361358

• Gene Names: ULK2; ATG1B; Unc51.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ULK2; Polyclonal Antibody; ID (S323) (ULK2; KIAA0623; Serine/threonine-protein kinase ULK2; Unc-51-like kinase 2) (FITC); Unc-51-like kinase 2; anti-ULK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ULK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-327 amino acids from the Central region of human ULK2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of U251 cell stained with ULK2 Antibody (Center S323).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ULK2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).ULK2 immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(ULK2 Antibody (S323) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ULK2 antibody detected the ULK2 protein (arrow).)

Related Product Information for anti-ULK2 antibody

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq].

Product Categories/Family for anti-ULK2 antibody

Antibodies; Protein Kinases

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009). Stelzl, U., et al. Cell 122(6):957-968(2005). Tomoda, T., et al. Genes Dev. 18(5):541-558(2004). Yan, J., et al. Oncogene 18(43):5850-5859(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 217330559
• NCBI GeneID: 9706
• NCBI Accession #: NP_001136082.1
• NCBI GenBank Nucleotide #: NM_001142610.1
• UniProt Accession #: Q8IYT8; O75119; A8MY69
• Molecular Weight: 112,694 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK2
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 2
• NCBI Official Symbol: ULK2
• NCBI Official Synonym Symbols: ATG1B; Unc51.2
• NCBI Protein Information: serine/threonine-protein kinase ULK2
• UniProt Protein Name: Serine/threonine-protein kinase ULK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK2
• UniProt Synonym Gene Names: KIAA0623
• UniProt Entry Name: ULK2_HUMAN

NCBI Description

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]

Uniprot Description

ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Kinase, protein; Autophagy; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; ULK family

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasmic vesicle membrane; pre-autophagosomal structure membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: response to starvation; axon extension; protein amino acid autophosphorylation; regulation of autophagy; negative regulation of collateral sprouting; signal transduction; autophagic vacuole formation