Rabbit anti-Human ULK2 Polyclonal Antibody | anti-ULK2 antibody

ULK2, NT (Serine/Threonine-protein Kinase ULK2, Unc-51-like Kinase 2, KIAA0623) (MaxLight 405)

Cat. Num. AAA6504798

• Gene Names: ULK2; ATG1B; Unc51.2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ULK2; Polyclonal Antibody; NT (Serine/Threonine-protein Kinase ULK2; Unc-51-like Kinase 2; KIAA0623) (MaxLight 405); anti-ULK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ULK2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-ULK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ULK2 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ULK2 Antibody (N-term) in K562 cell line lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ULK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ULK2 antibody

Antibodies; Protein Kinases

References

1. Yan, J., et al., Oncogene 18(43):5850-5859 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 217330557
• NCBI GeneID: 9706
• NCBI Accession #: NP_055498
• NCBI GenBank Nucleotide #: NM_014683.3
• UniProt Accession #: Q8IYT8; O75119; A8MY69
• Molecular Weight: Predicted: 105; 114 kDa; ; Observed: 100 kDa
• NCBI Official Full Name: serine/threonine-protein kinase ULK2
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 2
• NCBI Official Symbol: ULK2
• NCBI Official Synonym Symbols: ATG1B; Unc51.2
• NCBI Protein Information: serine/threonine-protein kinase ULK2
• UniProt Protein Name: Serine/threonine-protein kinase ULK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK2
• UniProt Synonym Gene Names: KIAA0623
• UniProt Entry Name: ULK2_HUMAN

NCBI Description

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]

Uniprot Description

ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Kinase, protein; Autophagy; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; ULK family

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasmic vesicle membrane; pre-autophagosomal structure membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: response to starvation; axon extension; protein amino acid autophosphorylation; regulation of autophagy; negative regulation of collateral sprouting; signal transduction; autophagic vacuole formation