Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cell using UIMC1 antibody. Green degree GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Rabbit anti-Human UIMC1 Polyclonal Antibody | anti-UIMC1 antibody
UIMC1 antibody
Immunofluorescence: 1:50 - 1:100
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cell using UIMC1 antibody. Green degree GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cell using UIMC1 antibody. Green degree GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using UIMC1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using UIMC1 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UIMC1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UIMC1 antibody.)
NCBI and Uniprot Product Information
Uniprot Description
RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 5q35.2
Cellular Component: nucleus
Molecular Function: retinoid X receptor binding; protein binding; histone binding
Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; negative regulation of transcription, DNA-dependent
Research Articles on UIMC1
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Product Notes
The UIMC1 uimc1 (Catalog #AAA9411681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UIMC1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UIMC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:100. Researchers should empirically determine the suitability of the UIMC1 uimc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UIMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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