Rabbit anti-Human UGT2B15 Polyclonal Antibody | anti-UGT2B15 antibody

UGT2B15, ID (UGT2B15, UGT2B8, UDP-glucuronosyltransferase 2B15, HLUG4, UDP-glucuronosyltransferase 2B8, UDPGTh-3) (Azide free) (HRP)

Cat. Num. AAA6361183

• Gene Names: UGT2B15; HLUG4; UGT2B8; UDPGTH3; UDPGT 2B8; UDPGT2B15
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UGT2B15; Polyclonal Antibody; ID (UGT2B15; UGT2B8; UDP-glucuronosyltransferase 2B15; HLUG4; UDP-glucuronosyltransferase 2B8; UDPGTh-3) (Azide free) (HRP); UDPGTh-3; anti-UGT2B15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UGT2B15.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UGT2B15 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 156-185 in the central region of human UGT2B15, UniProt Accession #P54855.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UGT2B15 Antibody (Center) western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).This demonstrates the UGT2B15 antibody detected the UGT2B15 protein (arrow).)

Immunohistochemistry (IHC)

(UGT2B15 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of UGT2B15 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(UGT2B15 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-UGT2B15 antibody

The UGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. UGT2B8 demonstrates reactivity with estriol.

Product Categories/Family for anti-UGT2B15 antibody

Antibodies; Enzymes; Glucuronosyltransferase

References

Yong, M., et al. Cancer Epidemiol. Biomarkers Prev. 19(2):537-546(2010). Sun, C., et al. Hum. Mutat. 31(1):99-107(2010). Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009). He, X., et al. Br J Clin Pharmacol 68(5):721-730(2009). Holmes, M.V., et al. PLoS ONE 4 (12), E7960 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 116517299
• NCBI GeneID: 7366
• NCBI Accession #: NP_001067.2
• NCBI GenBank Nucleotide #: NM_001076.3
• UniProt Accession #: P54855; P23765; Q9UK63; A6NDX0; A6NNJ4; AAB28536
• Molecular Weight: 61,036 Da
• NCBI Official Full Name: UDP-glucuronosyltransferase 2B15
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase 2 family, polypeptide B15
• NCBI Official Symbol: UGT2B15
• NCBI Official Synonym Symbols: HLUG4; UGT2B8; UDPGTH3; UDPGT 2B8; UDPGT2B15
• NCBI Protein Information: UDP-glucuronosyltransferase 2B15; UDPGTh-3; UDPGT 2B15; UDP glycosyltransferase 2B15; UDP-glucuronosyltransferase 2B8; UDP-glucuronosyltransferase UGT2B15; UDP-glucuronyltransferase, family 2, beta-15
• UniProt Protein Name: UDP-glucuronosyltransferase 2B15
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: UGT2B15
• UniProt Synonym Gene Names: UGT2B8; UDPGT 2B15; UDPGT 2B8
• UniProt Entry Name: UDB15_HUMAN

NCBI Description

This gene encodes a member of the UDP-glycosyltransferase (UDPGT) family. The UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This protein displays activity towards several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens. [provided by RefSeq, Oct 2011]

Uniprot Description

UGT2B15: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens. Belongs to the UDP-glycosyltransferase family.

Protein type: Transferase; EC 2.4.1.17; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - starch and sucrose; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: retinoic acid binding; glucuronosyltransferase activity

Biological Process: steroid metabolic process; flavonoid biosynthetic process; xenobiotic metabolic process; cellular response to hormone stimulus