Rabbit UGT1A3 Polyclonal Antibody | anti-UGT1A3 antibody

Anti-UGT1A3 Antibody

Cat. Num. AAA1753638

• Gene Names: UGT1A3; UDPGT; UGT1C; UGT-1C; UGT1.3; UGT1-03; UDPGT 1-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: UGT1A3; Polyclonal Antibody; Anti-UGT1A3 Antibody; GNT1; UGT1; UDP-glucuronosyltransferase 1-3; UDPGT 1-3; UGT1*3; UGT1-03; UGT1. 3; EC 2. 4. 1. 17; UDP-glucuronosyltransferase 1-C; UGT-1C; UGT1C; UDP-glucuronosyltransferase 1A3; UDP glucuronosyltransferase family 1 member A3; anti-UGT1A3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for UGT1A3 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-UGT1A3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml|Human, Mouse, Rat|; IHC-P: 2-5ug/ml|Human|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human UGT1A3 recombinant protein (Position: H92-C128).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-UGT1A3 antibody

UDP-glucuronosyltransferase 1-3 is an enzyme that in humans is encoded by the UGT1A3 gene. This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. Substrates of this enzyme include estrone, 2-hydroxyestrone, and metabolites of benzo alpha-pyrene.

References

1. Gong, Q. -H., Cho, J. W., Huang, T., Potter, C., Gholami, N., Basu, N. K., Kubota, S., Carvalho, S., Pennington, M. W., Owens, I. S., Popescu, N. C. Thirteen UDP-glucuronosyltransferase genes are encoded at the human UGT1 gene complex locus. Pharmacogenetics 11: 357-368, 2001.
2. Mackenzie, P. I., Bock, K. W., Burchell, B., Guillemette, C., Ikushiro, S., Iyanagi, T., Miners, J. O., Owens, I. S., Nebert, D. W. Nomenclature update for the mammalian UDP glycosyltransferase (UGT) gene superfamily. Pharmacogenet. Genomics 15: 677-685, 2005.
3. Moghrabi, N., Sutherland, L., Wooster, R., Povey, S., Boxer, M., Burchell, B. Chromosomal assignment of human phenol and bilirubin UDP glucuronosyltransferase genes (UGT1A-subfamily). Ann. Hum. Genet. 56: 81-91, 1992.

NCBI and Uniprot Product Information

• NCBI GI #: 13487900
• NCBI GeneID: 54659
• NCBI Accession #: NP_061966.1
• NCBI GenBank Nucleotide #: NM_019093.2
• UniProt Accession #: P35503
• Molecular Weight: 60,338 Da
• NCBI Official Full Name: UDP-glucuronosyltransferase 1-3
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase 1 family, polypeptide A3
• NCBI Official Symbol: UGT1A3
• NCBI Official Synonym Symbols: UDPGT; UGT1C; UGT-1C; UGT1.3; UGT1-03; UDPGT 1-3
• NCBI Protein Information: UDP-glucuronosyltransferase 1-3; UGT1*3; UDP-glucuronosyltransferase 1-C; UDP-glucuronosyltransferase 1A3; UDP glycosyltransferase 1 family, polypeptide A3
• UniProt Protein Name: UDP-glucuronosyltransferase 1-3
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: UGT1A3
• UniProt Synonym Gene Names: GNT1; UGT1; UDPGT 1-3; UGT1*3; UGT1-03; UGT1.3; UGT-1C; UGT1C
• UniProt Entry Name: UD13_HUMAN

NCBI Description

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. Substrates of this enzyme include estrone, 2-hydroxyestrone, and metabolites of benzo alpha-pyrene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.

Catalytic activity: UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

Subcellular location: Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein

Potential.

Sequence similarities: Belongs to the UDP-glycosyltransferase family.