Rabbit anti-Human, mouse UFSP2 Polyclonal Antibody | anti-UFSP2 antibody

UFSP2 Antibody

Cat. Num. AAA7004545

• Gene Names: UFSP2; BHD; C4orf20
• Reactivity: Human, mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: UFSP2; Polyclonal Antibody; UFSP2 Antibody; Ufm1-specific protease 2 (EC:3.4.22.-1 Publication; C4orf20; anti-UFSP2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Sequence Length: 469

• Applicable Applications for anti-UFSP2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
• Immunogen: Recombinant human Ufm1-specific protease 2 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-163500 / sc-163498 / sc-292068
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: UFSP2 antibody at 4ug/mlLane 1: HepG-2 whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Mouse kidney tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7004545 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7004545 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-UFSP2 antibody

Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 227498137
• NCBI GeneID: 55325
• NCBI Accession #: NP_060829.2
• NCBI GenBank Nucleotide #: NM_018359.3
• UniProt Accession #: Q9NUQ7; Q6IA77; Q96FS3
• Molecular Weight: 53,261 Da
• NCBI Official Full Name: ufm1-specific protease 2
• NCBI Official Synonym Full Names: UFM1-specific peptidase 2
• NCBI Official Symbol: UFSP2
• NCBI Official Synonym Symbols: BHD; C4orf20
• NCBI Protein Information: ufm1-specific protease 2
• UniProt Protein Name: Ufm1-specific protease 2
• Protein Family: Ufm1-specific protease
• UniProt Gene Name: UFSP2
• UniProt Synonym Gene Names: C4orf20; UfSP2
• UniProt Entry Name: UFSP2_HUMAN

NCBI Description

Like ubiquitin (see MIM 191339), ubiquitin-fold modifier-1 (UFM1; MIM 610553) must be processed by a protease before it can conjugate with its target proteins. UFSP2 is a thiol protease that specifically processes the C terminus of UFM1 (Kang et al., 2007 [PubMed 17182609]).[supplied by OMIM, Oct 2009]

Uniprot Description

UFSP2: Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, an ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Belongs to the peptidase C78 family.

Protein type: Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: protein binding; thiolester hydrolase activity

Biological Process: proteolysis; regulation of estrogen receptor signaling pathway

Disease: Beukes Hip Dysplasia