UCHL1 antibody pair

UCHL1 Antibody Pair

Cat. Num. AAA7041976

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Reactivity: Human, Mouse, Guinea Pig, Bovine, Horse, Pig, Dog, Goat, Sheep
• Applications: ELISA
• Synonyms: UCHL1; Polyclonal Antibody Pair; UCHL1 Antibody Pair; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1 antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Human, Mouse, Guinea Pig, Bovine, Horse, Pig, Dog, Goat, Sheep
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 223

• Applicable Applications for UCHL1 antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 1ug/ml.
• Immunogen Species: Human
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: PC-3 cell; A-549 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• Molecular Weight: 24,824 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Cell development/differentiation; Ligase

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum membrane; myelin sheath; nucleoplasm; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: adult walking behavior; axon target recognition; axon transport of mitochondrion; cell proliferation; eating behavior; muscle fiber development; negative regulation of MAP kinase activity; neuromuscular process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of macroautophagy; sensory perception of pain

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant