Western Blot (WB)
(The anti-UCHL5 Pab is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.)
Rabbit anti-Human, Mouse UCH37 Polyclonal Antibody | anti-UCH37 antibody
UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5) (AP)
Gene Names
UCHL5; UCH37; CGI-70; INO80R; UCH-L5
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
UCH37; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5; Ubiquitin Thiolesterase L5; Ubiquitin C-terminal Hydrolase UCH37; CGI-70; AD-019; UCHL5) (AP); anti-UCH37 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human UCHL5. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-UCH37 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human UCHL5 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-UCHL5 Pab is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.)
Western Blot (WB)
(The anti-UCHL5 Pab is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.)
Immunohistochemistry (IHC)
(HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A)correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.)
Immunohistochemistry (IHC)
(HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A)correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.)
Product Categories/Family for anti-UCH37 antibody
References
1. M-P. et al. Proteomics. July; 9(13): 3609-3622(2009). 2. Hu, R.M., et al., Proc. Natl. Acad. Sci. USA 97(17):9543-9548 (2000). 3. Lai, C.H., et al., Genome Res. 10(5):703-713 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39.7kDa (349aa), confirmed by MALDI-TOF
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L5
NCBI Official Symbol
UCHL5
NCBI Official Synonym Symbols
UCH37; CGI-70; INO80R; UCH-L5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L5
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L5
UniProt Gene Name
UCHL5
UniProt Synonym Gene Names
UCH37; UCH-L5
UniProt Entry Name
UCHL5_HUMAN
Research Articles on UCH37
Similar Products
UCH37 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, ELISA, Western Blot
UCH37 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, ELISA, Western Blot
Product Notes
The UCH37 uchl5 (Catalog #AAA6504694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UCH37 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UCH37 uchl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCH37, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
