Rabbit Ubiquitin Polyclonal Antibody | anti-UBB antibody

Ubiquitin Antibody: PerCP

Cat. Num. AAA806362

• Reactivity: Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
• Applications: Western Blot, Immunoprecipitation, Immunoprecipitation, Immunofluorescence
• Synonyms: Ubiquitin; Polyclonal Antibody; Ubiquitin Antibody: PerCP; Polyubiquitin B; RPS27A; UBA52; UBB; UBC; ubiquitin B; anti-UBB antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 305

• Applicable Applications for anti-UBB antibody: Western Blot (WB), Immunoprecipitation (IP), chIP, Immunofluorescence (IF)
• Application Notes: 1:1000-1500 (ECL)(WB)
• Conjugate: PerCP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Related Product Information for anti-UBB antibody

Background Info: The antibody recognizes ~10kDa corresponding to free ubiquitin of human, mouse, monkey, bovine and rat origins. It also recognizes ubiquinated proteins.

Scientific Background: Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic

Product Categories/Family for anti-UBB antibody

Apoptosis; Cell Signaling

References

1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 3. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57. 4. Boston Biochem: "Ubiquitin Proteasome Pathway Overview" http://www.bostonbiochem.com/upp.php 5. Yang M., et al. (1998) J Exp Med. 187: 1835-1846. 6. Chen Z.J., et al. (1996) Cell 84: 853-862.
Anne Bailey and Lorne J. Hofseth. A method to enhance the sensitivity and reproducibility of immunohistochemistry http://www.prohisto.com/pdfs/Hofseth_Manuscript-81.pdf

NCBI and Uniprot Product Information

• NCBI GI #: 27806505
• NCBI GeneID: 281370
• NCBI Accession #: NP_776558.1
• NCBI GenBank Nucleotide #: NM_174133.2
• UniProt Accession #: P0CG53; O97577; P02248; P02249; P02250; P62990; P80169; Q01235; Q24K23; Q28169; Q28170; Q29120
• Molecular Weight: 34,308 Da
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B<
• NCBI Official Symbol: UBB
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• Protein Family: Ubiquitin
• UniProt Gene Name: UBB
• UniProt Entry Name: UBB_BOVIN

Uniprot Description

UBB: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family.

Protein type: Cell cycle regulation; Cell development/differentiation; Transcription regulation; Apoptosis; Motility/polarity/chemotaxis; Ubiquitin-like modifier

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding