Rabbit anti-Human Ubiquitin +1 Polyclonal Antibody | anti-UBQ1 antibody

Ubiquitin +1

Cat. Num. AAA644064

• Gene Names: UBQ1; EARLY-RESPONSIVE TO DEHYDRATION 16; EMB2167; EMBRYO DEFECTIVE 2167; ERD16; F3C22.8; HAP4; HAPLESS 4; ubiquitin extension protein 1; UBQ1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Ubiquitin +1; Polyclonal Antibody; Anti -Ubiquitin +1; anti-UBQ1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Ubiquitin +1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

• Applicable Applications for anti-UBQ1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:5000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to human Ubiquitin +1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-UBQ1 antibody

Ubiquitin+1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially ub+1 is accumulated in aggregates containing amyloid-beta and phosphorylated-tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also ub +1 acts as an aggravating factor in polyglutamine-induced neurodegeneration.

Product Categories/Family for anti-UBQ1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 18409639
• NCBI GeneID: 824425
• NCBI Accession #: NP_566969.1
• NCBI GenBank Nucleotide #: NM_115119.3
• UniProt Accession #: B9DHA6; O80715; P19232; P59263; Q38875; Q9LDJ2; Q9LYW1; Q9M0W3; Q9M1P9; Q9S7X3
• Molecular Weight: 14,733 Da
• NCBI Official Full Name: ubiquitin extension protein 1
• NCBI Official Symbol: UBQ1
• NCBI Official Synonym Symbols: EARLY-RESPONSIVE TO DEHYDRATION 16; EMB2167; EMBRYO DEFECTIVE 2167; ERD16; F3C22.8; HAP4; HAPLESS 4; ubiquitin extension protein 1; UBQ1
• NCBI Protein Information: ubiquitin extension protein 1
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40-1
• UniProt Gene Name: RPL40A
• UniProt Synonym Gene Names: UBQ2
• UniProt Entry Name: RL40A_ARATH

NCBI Description

Ubiquitin extension protein

Uniprot Description

Function: Ubiquitin: exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling

By similarity.60S ribosomal protein L40-1: component of the 60S subunit of the ribosome.

Subunit structure: Ribosomal protein L40-1 is part of the 60S ribosomal subunit

By similarity.

Subcellular location: Ubiquitin: Cytoplasm

By similarity. Nucleus

By similarity. 60S ribosomal protein L40-1: Cytoplasm

By similarity.

Miscellaneous: Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino-acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to an ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.

Sequence similarities: In the N-terminal section; belongs to the ubiquitin family.In the C-terminal section; belongs to the ribosomal protein L40e family.Contains 1 ubiquitin-like domain.