Rabbit anti-Human UBE3A Polyclonal Antibody | anti-UBE3A antibody

Anti-UBE3A Antibody

Cat. Num. AAA1752942

• Gene Names: UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: UBE3A; Polyclonal Antibody; Anti-UBE3A Antibody; Ubiquitin-protein ligase E3A; E6AP ubiquitin-protein ligase; HECT-type ubiquitin transferase E3A; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; E6AP; EPVE6AP; HPVE6A; anti-UBE3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 875

• Applicable Applications for anti-UBE3A antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human); IHC-P (Embedded Section): 0.5-1ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human UBE3A recombinant protein (Position: M1-E860).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of UBE3A using anti-UBE3A antibody.)

Related Product Information for anti-UBE3A antibody

Description: Rabbit IgG polyclonal antibody for UBE3A detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.

Background: Ubiquitin-protein ligase E3A (UBE3A) also known as E6AP ubiquitin-protein ligase (E6AP) is an enzyme that in humans is encoded by the UBE3A gene. It is mapped to 15q11.2. This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

References

1. Abaied, L., Trabelsi, M., Chaabouni, M., Kharrat, M., Kraoua, L., M'rad, R., Tebib, N., Maazoul, F., Chaabouni, H. A novel UBE3A truncating mutation in large Tunisian Angelman syndrome pedigree. Am. J. Med. Genet. 152A: 141-146, 2010.
2. Albrecht, U., Sutcliffe, J. S., Cattanach, B. M., Beechey, C. V., Armstrong, D., Eichele, G., Beaudet, A. L. Imprinted expression of the murine Angelman syndrome gene, Ube3a, in hippocampal and Purkinje neurons. Nature Genet. 17: 75-78, 1997.
3. Camprubi, C., Guitart, M., Gabau, E., Coll, M. D., Villatoro, S., Oltra, S., Rosello, M., Ferrer, I., Monfort, S., Orellana, C., Martinez, F. Novel UBE3A mutations causing Angelman syndrome: different parental origin for single nucleotide changes and multiple nucleotide deletions or insertions. Am. J. Med. Genet. 149A: 343-348, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 19718766
• NCBI GeneID: 7337
• NCBI Accession #: NP_000453.2
• NCBI GenBank Nucleotide #: NP_000453.2
• UniProt Accession #: Q05086
• NCBI Official Full Name: ubiquitin-protein ligase E3A isoform 2
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3A
• NCBI Official Symbol: UBE3A
• NCBI Official Synonym Symbols: AS; ANCR; E6-AP; HPVE6A; EPVE6AP
• NCBI Protein Information: ubiquitin-protein ligase E3A
• UniProt Protein Name: Ubiquitin-protein ligase E3A
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3A
• UniProt Synonym Gene Names: E6AP; EPVE6AP; HPVE6A
• UniProt Entry Name: UBE3A_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]