Rabbit anti-Human UBE3A Polyclonal Antibody | anti-UBE3A antibody

UBE3A, CT (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, Oncogenic Protein-associated Protein E6-AP, Human Papillomavirus E6-associated Protein, Renal Carcinoma Antigen NY-REN-54, E6AP, EPVE6AP, HPVE6A) (AP)

Cat. Num. AAA6473430

• Gene Names: UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: UBE3A; Polyclonal Antibody; CT (Ubiquitin-protein Ligase E3A; E6AP Ubiquitin-protein Ligase; Oncogenic Protein-associated Protein E6-AP; Human Papillomavirus E6-associated Protein; Renal Carcinoma Antigen NY-REN-54; E6AP; EPVE6AP; HPVE6A) (AP); anti-UBE3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE3A.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-UBE3A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa843-873 from the C-terminal region of human UBE3A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UBE3A Antibody (C-term) in T47D cell line lysates (35ug/lane).UBE3A(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of UBE3A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(UBE3A Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-UBE3A antibody

Antibodies; Ubiquitin Ligases

References

1. Be, X., et al., Biochemistry 40(5):1293-1299 (2001). 2. Kleijnen, M.F., et al., Mol. Cell 6(2):409-419 (2000). 3. Huang, L., et al., Science 286(5443):1321-1326 (1999). 4. Nuber, U., et al., Eur. J. Biochem. 254(3):643-649 (1998). 5. Malzac, P., et al., Am. J. Hum. Genet. 62(6):1353-1360 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 19718766
• NCBI GeneID: 7337
• UniProt Accession #: Q05086; P78355; Q93066; Q9UEP4; Q9UEP5; Q9UEP6; Q9UEP7; Q9UEP8; Q9UEP9; A8K8Z9
• NCBI Official Full Name: ubiquitin-protein ligase E3A isoform 2 [Homo sapiens]
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3A
• NCBI Official Symbol: UBE3A
• NCBI Official Synonym Symbols: AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
• UniProt Protein Name: Ubiquitin-protein ligase E3A
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3A
• UniProt Synonym Gene Names: E6AP; EPVE6AP; HPVE6A
• UniProt Entry Name: UBE3A_HUMAN