Rabbit anti-Human, Mouse UBE2U Polyclonal Antibody | anti-UBE2U antibody

UBE2U Polyclonal Antibody

Cat. Num. AAA3009157

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE2U; Polyclonal Antibody; UBE2U Polyclonal Antibody; Ubiquitin-conjugating enzyme E2 U; E2 ubiquitin-conjugating enzyme U; Ubiquitin carrier protein U; Ubiquitin-protein ligase U; anti-UBE2U antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE2U polyclonal antibody detects endogenous levels of UBE2U protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 226

• Applicable Applications for anti-UBE2U antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human UBE2U.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using UBE2U antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-UBE2U antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3, 000 genes and comprises nearly 8% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 22749027
• NCBI GeneID: 148581
• NCBI Accession #: NP_689702.1
• NCBI GenBank Nucleotide #: NP_689702.1
• UniProt Accession #: Q5VVX9
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 U isoform 4
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 U
• NCBI Official Symbol: UBE2U
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 U
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 U
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2U
• UniProt Entry Name: UBE2U_HUMAN

Uniprot Description

Function: Catalyzes the covalent attachment of ubiquitin to other proteins

By similarity.

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.