Rabbit anti-Human UBE2S Polyclonal Antibody | anti-UBE2S antibody

UBE2S, CT (UBE2S, E2EPF, Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24kD, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S) (FITC)

Cat. Num. AAA6360797

• Gene Names: UBE2S; EPF5; E2EPF; E2-EPF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2S; Polyclonal Antibody; CT (UBE2S; E2EPF; Ubiquitin-conjugating enzyme E2 S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24kD; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S) (FITC); Ubiquitin-protein ligase S; anti-UBE2S antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-UBE2S antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2S antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the C-terminal region of human UBE2S.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBE2S Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the UBE2S antibody detected the UBE2S protein (arrow).)

Related Product Information for anti-UBE2S antibody

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

Product Categories/Family for anti-UBE2S antibody

Antibodies; Ubiquitin Ligases

References

Wu, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1355-1360(2010). Garnett, M.J., et al. Nat. Cell Biol. 11(11):1363-1369(2009). Williamson, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(43):18213-18218(2009). Chen, M.F., et al. J. Mol. Med. 87(3):307-320(2009). Lim, J.H., et al. J. Cell. Biochem. 105(4):1117-1127(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 112382377
• NCBI GeneID: 27338
• NCBI Accession #: NP_055316.2
• NCBI GenBank Nucleotide #: NM_014501.2
• UniProt Accession #: Q16763; Q9BTC1
• Molecular Weight: 23,845 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 S
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2S
• NCBI Official Symbol: UBE2S
• NCBI Official Synonym Symbols: EPF5; E2EPF; E2-EPF
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 S; E2-EPF5; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-protein ligase S
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 S
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2S
• UniProt Synonym Gene Names: E2EPF
• UniProt Entry Name: UBE2S_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2S: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: anaphase-promoting complex; cytoplasm

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; protein modification process; exit from mitosis