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Western Blot (WB) (UBE2Q1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the UBE2Q1 antibody detected the UBE2Q1 protein (arrow).)

Rabbit anti-Human UBE2Q1 Polyclonal Antibody | anti-UBE2Q1 antibody

UBE2Q1, NT (UBE2Q1, NICE5, UBE2Q, Ubiquitin-conjugating enzyme E2 Q1, Protein NICE-5, Ubiquitin carrier protein Q1, Ubiquitin-protein ligase Q1) (Azide free) (HRP)

Gene Names
UBE2Q1; GTAP; UBE2Q; NICE-5; PRO3094
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
UBE2Q1; Polyclonal Antibody; NT (UBE2Q1; NICE5; UBE2Q; Ubiquitin-conjugating enzyme E2 Q1; Protein NICE-5; Ubiquitin carrier protein Q1; Ubiquitin-protein ligase Q1) (Azide free) (HRP); Ubiquitin-protein ligase Q1; anti-UBE2Q1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-UBE2Q1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
UBE2Q1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-104 amino acids from the N-terminal region of human UBE2Q1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBE2Q1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the UBE2Q1 antibody detected the UBE2Q1 protein (arrow).)

Western Blot (WB) (UBE2Q1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the UBE2Q1 antibody detected the UBE2Q1 protein (arrow).)
Product Categories/Family for anti-UBE2Q1 antibody
References
Wassler, M.J., et al. Stem Cells 26(8):2006-2018(2008). Grelle, G., et al. Mol. Cell Proteomics 5(2):234-244(2006). Wistow, G., et al. Mol. Vis. 8, 205-220 (2002): Marenholz, I., et al. Genome Res. 11(3):341-355(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,127 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 Q1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2Q family member 1
NCBI Official Symbol
UBE2Q1
NCBI Official Synonym Symbols
GTAP; UBE2Q; NICE-5; PRO3094
NCBI Protein Information
ubiquitin-conjugating enzyme E2 Q1; protein NICE-5; ubiquitin-protein ligase Q1; ubiquitin carrier protein Q1; galactosyl transferase-associated protein; ubiquitin-conjugating enzyme E2Q (putative) 1
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 Q1
UniProt Gene Name
UBE2Q1
UniProt Synonym Gene Names
NICE5; UBE2Q
UniProt Entry Name
UB2Q1_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is 98% identical to the mouse counterpart. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2Q1: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleus; cytosol; filopodium

Molecular Function: protein binding; ATP binding; ligase activity

Biological Process: fertilization; suckling behavior; mating behavior; protein ubiquitination; embryo implantation

Research Articles on UBE2Q1

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Product Notes

The UBE2Q1 ube2q1 (Catalog #AAA6360765) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2Q1, NT (UBE2Q1, NICE5, UBE2Q, Ubiquitin-conjugating enzyme E2 Q1, Protein NICE-5, Ubiquitin carrier protein Q1, Ubiquitin-protein ligase Q1) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2Q1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UBE2Q1 ube2q1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2Q1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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