Rabbit anti-Human UBE2L3 Polyclonal Antibody | anti-UBE2L3 antibody

UBE2L3, CT (UBE2L3, UBCE7, UBCH7, Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3) (FITC)

Cat. Num. AAA6360731

• Gene Names: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4; UBE2L3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2L3; Polyclonal Antibody; CT (UBE2L3; UBCE7; UBCH7; Ubiquitin-conjugating enzyme E2 L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3) (FITC); Ubiquitin-protein ligase L3; anti-UBE2L3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-UBE2L3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBE2L3 Antibody (C-term) western blot analysis in K562,U87-MG,mouse NIH/3T3,rat PC-12 cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the UBE2L3 antibody detected the UBE2L3 protein (arrow).)

Product Categories/Family for anti-UBE2L3 antibody

Antibodies; Ubiquitin Ligases

References

Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010). Purbeck, C., et al. Biochemistry 49(7):1361-1363(2010). Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507789
• NCBI GeneID: 7332
• UniProt Accession #: P68036; P51966; P70653; Q9HAV1; B2R4A7
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 L3 [Homo sapiens]
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 3
• NCBI Official Symbol: UBE2L3
• NCBI Official Synonym Symbols: E2-F1; L-UBC; UBCH7; UbcM4; UBE2L3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2L3
• UniProt Synonym Gene Names: UBCE7; UBCH7
• UniProt Entry Name: UB2L3_HUMAN

Uniprot Description

UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein modification process; protein ubiquitination