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Western Blot (WB) (The anti-UBCH9 Pab is used in Western blot to detect UBCH9 in Jurkat cell lysate.)

Rabbit anti-Human UBCH9 Polyclonal Antibody | anti-UBCH9 antibody

UBCH9, CT (Ubiquitin-conjugating Enzyme E2 E3, Ubiquitin-protein Ligase E3, Ubiquitin Carrier Protein E3, Ubiquitin-conjugating Enzyme E2-23kD, UbcH9, UBE2E3, UBCE4) (FITC)

Gene Names
UBE2E3; UBCH9; UbcM2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
UBCH9; Polyclonal Antibody; CT (Ubiquitin-conjugating Enzyme E2 E3; Ubiquitin-protein Ligase E3; Ubiquitin Carrier Protein E3; Ubiquitin-conjugating Enzyme E2-23kD; UbcH9; UBE2E3; UBCE4) (FITC); anti-UBCH9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human UBCH9.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-UBCH9 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human UBCH9 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-UBCH9 Pab is used in Western blot to detect UBCH9 in Jurkat cell lysate.)

Western Blot (WB) (The anti-UBCH9 Pab is used in Western blot to detect UBCH9 in Jurkat cell lysate.)
Product Categories/Family for anti-UBCH9 antibody
References
1. Ito, K., et al., Cytogenet. Cell Genet. 84(1-2), 99-104 (1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,913 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 E3
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 E3
NCBI Official Symbol
UBE2E3
NCBI Official Synonym Symbols
UBCH9; UbcM2
NCBI Protein Information
ubiquitin-conjugating enzyme E2 E3
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 E3
UniProt Gene Name
UBE2E3
UniProt Synonym Gene Names
UBCE4; UBCH9
UniProt Entry Name
UB2E3_HUMAN

Uniprot Description

UBE2E3: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2q32.1

Molecular Function: protein binding; ubiquitin-protein ligase activity

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Product Notes

The UBCH9 ube2e3 (Catalog #AAA6504389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBCH9, CT (Ubiquitin-conjugating Enzyme E2 E3, Ubiquitin-protein Ligase E3, Ubiquitin Carrier Protein E3, Ubiquitin-conjugating Enzyme E2-23kD, UbcH9, UBE2E3, UBCE4) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBCH9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UBCH9 ube2e3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBCH9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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