Rabbit anti-Human UBA7 Polyclonal Antibody | anti-UBA7 antibody

UBA7 (Ubiquitin-like Modifier-activating Enzyme 7, Ubiquitin-activating Enzyme 7, D8, Ubiquitin-activating Enzyme E1 Homolog, UBE1L, UBE2, UBA1B) (HRP)

Cat. Num. AAA6397802

• Gene Names: UBA7; D8; UBE2; UBA1B; UBE1L
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBA7; Polyclonal Antibody; UBA7 (Ubiquitin-like Modifier-activating Enzyme 7; Ubiquitin-activating Enzyme 7; D8; Ubiquitin-activating Enzyme E1 Homolog; UBE1L; UBE2; UBA1B) (HRP); anti-UBA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBA7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UBA7 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human UBA7, aa1-1012 (NP_003326.2).
• Immunogen Sequence: MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBA7 rabbit polyclonal antibody. Western Blot analysis of UBA7 expression in human stomach.)

Western Blot (WB)

(Western Blot analysis of UBA7 expression in transfected 293T cell line by UBA7 polyclonal antibody. Lane 1: UBA7 transfected lysate (111.7kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-UBA7 antibody

Antibodies; Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 38045948
• NCBI GeneID: 7318
• NCBI Accession #: NP_003326.2
• NCBI GenBank Nucleotide #: NM_003335.2
• UniProt Accession #: P41226; Q9BRB2
• Molecular Weight: 111,694 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 7
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 7
• NCBI Official Symbol: UBA7
• NCBI Official Synonym Symbols: D8; UBE2; UBA1B; UBE1L
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 7; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme-2; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme E1 homolog; UBA1, ubiquitin-activating enzyme E1 homolog B; ubiquitin-activating e
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: UBA7
• UniProt Synonym Gene Names: UBE1L; UBE2; Ubiquitin-activating enzyme 7
• UniProt Entry Name: UBA7_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1L: Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: cytosol; nucleus

Molecular Function: ISG15 activating enzyme activity; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding

Biological Process: ISG15-protein conjugation; modification-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; innate immune response; protein modification process; protein ubiquitination; negative regulation of interferon type I production