Rabbit UBA52 Polyclonal Antibody | anti-UBA52 antibody

UBA52 Antibody

Cat. Num. AAA9417246

• Gene Names: UBA52; L40; CEP52; RPL40; HUBCEP52
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: UBA52; Polyclonal Antibody; UBA52 Antibody; L40; CEP52; RPL40; HUBCEP52; anti-UBA52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total UBA52 protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 128

• Applicable Applications for anti-UBA52 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot: 1:200-1000; Immunohistochemistry: 1: 20-100
• Immunogen: Synthetic peptide of human UBA52
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C

SDS-Page

(Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-2: 293T and Raji cell lysates, Primary antibody:UBA52 antibody at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using UBA52 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200))

Related Product Information for anti-UBA52 antibody

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Product Categories/Family for anti-UBA52 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 77539055
• NCBI GeneID: 7311
• NCBI Accession #: NP_001029102.1
• NCBI GenBank Nucleotide #: NM_001033930.2
• UniProt Accession #: P62987; P02248; P02249; P02250; P14793; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888
• Molecular Weight: 14,728 Da
• NCBI Official Full Name: ubiquitin-60S ribosomal protein L40 isoform 1
• NCBI Official Synonym Full Names: ubiquitin A-52 residue ribosomal protein fusion product 1
• NCBI Official Symbol: UBA52
• NCBI Official Synonym Symbols: L40; CEP52; RPL40; HUBCEP52
• NCBI Protein Information: ubiquitin-60S ribosomal protein L40
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40
• UniProt Gene Name: UBA52
• UniProt Synonym Gene Names: UBCEP2
• UniProt Entry Name: RL40_HUMAN

NCBI Description

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

UBA52: the gene (UBA52) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 60S ribosomal protein L40 (77 ? 128). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 19p13.1-p12

Cellular Component: cytosol; endosome membrane; extracellular space; lysosomal membrane; nucleoplasm; nucleus; plasma membrane

Molecular Function: protein binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; bypass DNA synthesis; cellular protein metabolic process; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endosome transport; error-prone postreplication DNA repair; fibroblast growth factor receptor signaling pathway; G2/M transition of mitotic cell cycle; glycogen biosynthetic process; I-kappaB kinase/NF-kappaB cascade; innate immune response; JNK cascade; macroautophagy; MAPKKK cascade; mRNA catabolic process, nonsense-mediated decay; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of interferon type I production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; Notch signaling pathway; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; positive regulation of apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein modification process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of interferon type I production; regulation of mRNA stability; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; transcription-coupled nucleotide-excision repair; transforming growth factor beta receptor signaling pathway; translational initiation; tumor necrosis factor-mediated signaling pathway; viral infectious cycle; viral transcription; virus assembly; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway