Rabbit anti-Mouse Tyrosyl DNA Phosphodiesterase 1 Polyclonal Antibody | anti-TDP1 antibody

Polyclonal Antibody to Tyrosyl DNA Phosphodiesterase 1 (TDP1)

Cat. Num. AAA2033870

• Gene Names: Tdp1; SCAN1; MLZ-501; AI838772; AW493413; 2810481F14Rik; 4921509N21Rik; E430034L06Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Tyrosyl DNA Phosphodiesterase 1; Polyclonal Antibody; Polyclonal Antibody to Tyrosyl DNA Phosphodiesterase 1 (TDP1); SCAN1; Tyr-DNA phosphodiesterase 1; anti-TDP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TDP1. It has been selected for its ability to recognize TDP1 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 608

• Applicable Applications for anti-TDP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-1:5000
• Fragment: TDP1 (Glu4~Gln215)
• Conjugate: No Conjugate
• Cross Reactivity: Mouse
• Immunoglobulin Type: IgG
• Quality Control: Content: The quality control contains recombinant TDP1 (Glu4~Gln215) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2068196
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded breast tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 37999670
• NCBI GeneID: 104884
• UniProt Accession #: Q8BJ37; Q8VE39
• Molecular Weight: 68,689 Da
• NCBI Official Full Name: Tyrosyl-DNA phosphodiesterase 1
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Symbol: Tdp1
• NCBI Official Synonym Symbols: SCAN1; MLZ-501; AI838772; AW493413; 2810481F14Rik; 4921509N21Rik; E430034L06Rik
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 1
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 1
• Protein Family: Tyrosyl-DNA phosphodiesterase
• UniProt Gene Name: Tdp1
• UniProt Synonym Gene Names: Tyr-DNA phosphodiesterase 1; MLZ-501

Uniprot Description

TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.

Protein type: DNA repair, damage; EC 3.1.4.-; Phosphodiesterase

Chromosomal Location of Human Ortholog: 12|12 E

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: double-stranded DNA binding; single-stranded DNA binding; tyrosyl-DNA phosphodiesterase activity

Biological Process: DNA repair; double-strand break repair; single strand break repair