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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TYMP antibody.)

Rabbit TYMP Polyclonal Antibody | anti-TYMP antibody

TYMP (Thymidine Phosphorylase, ECGF1, MNGIE, PDECGF, TP, hPD-ECGF) (HRP)

Gene Names
TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
TYMP; Polyclonal Antibody; TYMP (Thymidine Phosphorylase; ECGF1; MNGIE; PDECGF; TP; hPD-ECGF) (HRP); anti-TYMP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes TYMP. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-TYMP antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human TYMP.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TYMP antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TYMP antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach cancer using TYMP antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using TYMP antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using TYMP antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-TYMP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,955 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1 proprotein
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
NCBI Protein Information
thymidine phosphorylase; tdRPase; gliostatin
UniProt Protein Name
Thymidine phosphorylase
UniProt Gene Name
TYMP
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF
UniProt Entry Name
TYPH_HUMAN

NCBI Description

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

Uniprot Description

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Phosphorylase; EC 2.4.2.4; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Cytokine; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; DNA replication

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; chemotaxis; DNA replication; cell differentiation

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)

Research Articles on TYMP

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Product Notes

The TYMP tymp (Catalog #AAA6442098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TYMP (Thymidine Phosphorylase, ECGF1, MNGIE, PDECGF, TP, hPD-ECGF) (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TYMP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TYMP tymp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TYMP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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