Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TXNDC5 Polyclonal Antibody | anti-TXNDC5 antibody
Goat anti-TXNDC5 (aa281-295) Antibody
Gene Names
TXNDC5; HCC2; ERP46; HCC-2; STRF8; PDIA15; UNQ364; ENDOPDI
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TXNDC5; Polyclonal Antibody; Goat anti-TXNDC5 (aa281-295) Antibody; Goat Anti-TXNDC5 (aa269-282) Antibody; thioredoxin domain containing 5 (endoplasmic reticulum); ERP46; EndoPDI; Hcc-2; MGC3178; PDIA15; UNQ364; ER protein 46; endoplasmic reticulum protein ERp46; endoplasmic reticulum resident protein 46; endothelial protein disulphide isomerase; prote; TXNDC5 antibody; thioredoxin domain containing 5 (endoplasmic reticulum) antibody; ERP46 antibody; EndoPDI antibody; Hcc-2 antibody; MGC3178 antibody; PDIA15 antibody; UNQ364 antibody; ER protein 46 antibody; endoplasmic reticulum protein ERp46 antibody; endoplasmic reticulum resident protein 46 antibody; endothelial protein disulphide isomerase antibody; protein disulfide isomerase family A; member 15 antibody; thioredoxin domain-containing protein 5 antibody; thioredoxin related protein antibody; thioredoxin-like protein p46 antibody; TXNDC5 (aa281-295); anti-TXNDC5 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RDLESLREYVESQLQ
Sequence Length
432
Applicable Applications for anti-TXNDC5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-RDLESLREYVESQLQ, from the internal region of the protein sequence according to NP_110437.2; NP_001139021.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Lymph Nodes (A) and Mouse Intestine (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Lymph Nodes (A) and Mouse Intestine (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,178 Da
NCBI Official Full Name
thioredoxin domain-containing protein 5 isoform 1
NCBI Official Synonym Full Names
thioredoxin domain containing 5
NCBI Official Symbol
TXNDC5
NCBI Official Synonym Symbols
HCC2; ERP46; HCC-2; STRF8; PDIA15; UNQ364; ENDOPDI
NCBI Protein Information
thioredoxin domain-containing protein 5
UniProt Protein Name
Thioredoxin domain-containing protein 5
Protein Family
UniProt Gene Name
TXNDC5
UniProt Synonym Gene Names
TLP46; ER protein 46; ERp46
UniProt Entry Name
TXND5_HUMAN
NCBI Description
This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq, Dec 2010]
Uniprot Description
TXNDC5: Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide- isomerase deficiency in yeast. Belongs to the protein disulfide isomerase family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6p24.3
Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; lysosomal lumen
Molecular Function: protein binding; protein disulfide isomerase activity
Biological Process: apoptotic cell clearance; cell redox homeostasis; metabolic process; negative regulation of apoptosis; protein folding
Research Articles on TXNDC5
Similar Products
Product Notes
The TXNDC5 txndc5 (Catalog #AAA422858) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TXNDC5 (aa281-295) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TXNDC5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the TXNDC5 txndc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDLESLREYV ESQLQ. It is sometimes possible for the material contained within the vial of "TXNDC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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