Western Blot (WB)
(Western blot analysis of Phospho-TXN(Thr100) in lysates of HeLa , using Phospho-TXN(Thr100) Antibody.)
Rabbit TXN Polyclonal Antibody | anti-TXN antibody
Phospho-TXN (Thr100) Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit
Applications
Western Blot, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
TXN; Polyclonal Antibody; Phospho-TXN (Thr100) Antibody; ADF; ATL derived factor; ATL-derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; testicular tissue protein Li 199; THIO_HUMAN; Thioredoxin; thioredoxin delta 3; TRDX; TRX 1; Trx; TRX1; TXN delta 3; TXN protein; zgc:92903; anti-TXN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit
Predicted: Pig, Bovine, Horse, Sheep, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-TXN(Thr100) Antibody detects endogenous levels of TXN only when phosphorylated at Thr100.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
85
Applicable Applications for anti-TXN antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA (Peptide): 1:20000-1:40000
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human TXN around the phosphorylation site of Thr100.
Tissue Specificity
Up-regulated by ionizing radiation.
Subcellular Location
Extracellular Region or Secreted; Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Phospho-TXN(Thr100) in lysates of HeLa , using Phospho-TXN(Thr100) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-TXN(Thr100) in lysates of HeLa , using Phospho-TXN(Thr100) Antibody.)
Related Product Information for anti-TXN antibody
Description: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Post Translational Modifications: In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Subunit Structure: Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.
Similarity: Belongs to the thioredoxin family.
Post Translational Modifications: In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Subunit Structure: Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.
Similarity: Belongs to the thioredoxin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 12 kDa
Predicted: 12 kDa
Predicted: 12 kDa
NCBI Official Full Name
thioredoxin isoform 2
NCBI Official Synonym Full Names
thioredoxin
NCBI Official Symbol
TXN
NCBI Official Synonym Symbols
TRX; TRDX; TRX1
NCBI Protein Information
thioredoxin
UniProt Protein Name
Thioredoxin
UniProt Gene Name
TXN
UniProt Synonym Gene Names
TRDX; TRX; TRX1; Trx; ADF; SASP
UniProt Entry Name
THIO_HUMAN
NCBI Description
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on TXN
1. Trx-1 may be a promising prognostic indicator and therapeutic target for gastric cancer patients.
Similar Products
Product Notes
The TXN txn (Catalog #AAA9610439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TXN (Thr100) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's TXN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the TXN txn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TXN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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