Rabbit anti-Human, Mouse TXK Polyclonal Antibody | anti-TXK antibody

TXK Antibody

Cat. Num. AAA9603004

• Gene Names: TXK; RLK; TKL; BTKL; PTK4; PSCTK5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TXK; Polyclonal Antibody; TXK Antibody; BTKL; EC 2.7.10.2; MGC22473; Protein-tyrosine kinase 4; PSCTK5; PTK4; Resting lymphocyte kinase; RLK; TKL; TXK tyrosine kinase; TXK_HUMAN; Tyrosine protein kinase TXK; Tyrosine-protein kinase TXK; anti-TXK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TXK antibody detects endogenous levels of total TXK
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 527

• Applicable Applications for anti-TXK antibody: Western Blot (WB), Immunofluorescence/Immunocytochemistry (IF/ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500; ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human TXK
• Subcellular Location: Cytoplasm. Nucleus. Cell Membrane. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.
• Tissue Specificity: Expressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential.
• Conjugation: Unconjugated
• Immportant: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western blot (WB)

(Western blot analysis of extracts from various samples, using TXK Ab.Lane 1: Mouse brain, treated with blocking peptideLane 2: Mouse brainLane 3: HepG2.Observed bands: 70 kDa.)

Western blot (WB)

(Western blot analysis of TXK using HeLa whole cell lysates)

Immunofluorescence

(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.)

Related Product Information for anti-TXK antibody

Function: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
Subunit Structure: Interacts with PARP1 and EEF1A1. Interacts with SH2D2A (By similarity).
Post-translational Modifications: Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription. The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 148596974
• NCBI GeneID: 7294
• NCBI Accession #: NP_003319.2
• NCBI GenBank Nucleotide #: NM_003328.2
• UniProt Accession #: P42681; Q14220
• Molecular Weight: 61 kDa
• NCBI Official Full Name: tyrosine-protein kinase TXK
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: TXK
• NCBI Official Synonym Symbols: RLK; TKL; BTKL; PTK4; PSCTK5
• NCBI Protein Information: tyrosine-protein kinase TXK
• UniProt Protein Name: Tyrosine-protein kinase TXK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: TXK
• UniProt Synonym Gene Names: PTK4; RLK

Uniprot Description

Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.