Western Blot (WB)
(All lanes: Twinfilin-2 antibody at 3ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateLane 3:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Rabbit anti-Human Twinfilin-2 Polyclonal Antibody | anti-TWF2 antibody
Rabbit anti-human Twinfilin-2 polyclonal Antibody(TWF2)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: Twinfilin-2 antibody at 3ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateLane 3:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Western Blot (WB)
(All lanes: Twinfilin-2 antibody at 3ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateLane 3:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using MBS1494408 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using MBS1494408 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating TWF2 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1494408 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1494408(4ug)+ HepG2 whole cell lysate(500ug)Lane 3:HepG2 whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating TWF2 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1494408 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1494408(4ug)+ HepG2 whole cell lysate(500ug)Lane 3:HepG2 whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]
Uniprot Description
A6r: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: cytoplasm; cytoskeleton; filopodium; growth cone; lamellipodium; myofibril; perinuclear region of cytoplasm; stereocilium
Molecular Function: actin monomer binding; ATP binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein kinase C binding
Biological Process: barbed-end actin filament capping; cell projection organization and biogenesis; negative regulation of actin filament polymerization; positive regulation of axon extension; regulation of actin cytoskeleton organization and biogenesis; regulation of microvillus length; sequestering of actin monomers
Research Articles on TWF2
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Product Notes
The TWF2 twf2 (Catalog #AAA1494408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Twinfilin-2 polyclonal Antibody(TWF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Twinfilin-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the TWF2 twf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Twinfilin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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