Rabbit anti-Human Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) Polyclonal Antibody | anti-TNFSF14 antibody

Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)

Cat. Num. AAA2006497

• Gene Names: TNFSF14; LTg; CD258; HVEML; LIGHT
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Tumor Necrosis Factor Ligand Superfamily; Member 14 (TNFSF14); Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily; anti-TNFSF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TNFSF14. It has been selected for its ability to recognize TNFSF14 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- HWRLGEMVT RLPDGPAGSW EQLIQERRSH EVNPAAHLTG ANSSLTGSGG PLLWETQLGL AFLRGLSYHD GALVVTKAGY YYIYSKVQLG GVGCPLGLAS TITHGLYKRT PRYPEELELL VSQQSPCGRA TSSSRVWWDS SFLGGVVHLE AGEKVVVRVL DERLVRLRDG TRSYFGAFMV
• Sequence Length: 240

• Applicable Applications for anti-TNFSF14 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant TNFSF14 (His62~Val240) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2048224
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant TNFSF14, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Stomach Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Liver Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Liver Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 25952144
• NCBI GeneID: 8740
• NCBI Accession #: NP_003798.2
• NCBI GenBank Nucleotide #: NM_003807.4
• UniProt Accession #: O43557; O75476; Q6FHA1; Q8WVF8; Q96LD2; A8K7M2; C9J5H4
• Molecular Weight: 22,395 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 14 isoform 1
• NCBI Official Synonym Full Names: TNF superfamily member 14
• NCBI Official Symbol: TNFSF14
• NCBI Official Synonym Symbols: LTg; CD258; HVEML; LIGHT
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 14
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 14
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF14
• UniProt Synonym Gene Names: HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; extracellular space; integral component of membrane; plasma membrane

Molecular Function: caspase inhibitor activity; cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding

Biological Process: apoptosis; immune response; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of myoblast differentiation; release of cytoplasmic sequestered NF-kappaB; signal transduction; T cell activation; T cell costimulation; T cell homeostasis; T cell proliferation; tumor necrosis factor-mediated signaling pathway