Western Blot (WB)
(Western blot analysis of TUFM Antibody (N-term) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.)
Rabbit anti-Human TUFM Polyclonal Antibody | anti-TUFM antibody
TUFM, NT (Elongation Factor Tu, Mitochondrial, EF-Tu, P43) (MaxLight 405)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of TUFM Antibody (N-term) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of TUFM Antibody (N-term) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TUFM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TUFM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(TUFM Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TUFM Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.)
Western Blot (WB)
(Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.)
NCBI and Uniprot Product Information
Uniprot Description
EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Protein type: Mitochondrial; RNA-binding; Translation elongation; Translation
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: mitochondrion; membrane
Molecular Function: GTPase activity; protein binding; GTP binding; translation elongation factor activity
Biological Process: translational elongation; mitochondrial translation; organelle organization and biogenesis
Disease: Combined Oxidative Phosphorylation Deficiency 4
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Product Notes
The TUFM tufm (Catalog #AAA6473590) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUFM, NT (Elongation Factor Tu, Mitochondrial, EF-Tu, P43) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TUFM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TUFM tufm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUFM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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