Western Blot (WB)
(Western Blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain)
Rabbit Tubulingamma Polyclonal Antibody | anti-TUBG1 antibody
Tubulingamma Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain)
Western Blot (WB)
(Western Blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain)
Immunocytochemistry (ICC)
(ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
Uniprot Description
TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: centriole; centrosome; recycling endosome; pericentriolar material; condensed nuclear chromosome; cytoplasmic microtubule; nonmotile primary cilium; apical part of cell; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton
Biological Process: metabolic process; organelle organization and biogenesis; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; G2/M transition of mitotic cell cycle; cytoplasmic microtubule organization and biogenesis; meiotic spindle organization and biogenesis
Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4
Research Articles on TUBG1
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Product Notes
The TUBG1 tubg1 (Catalog #AAA3013617) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulingamma Polyclonal Antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulingamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TUBG1 tubg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulingamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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