Western Blot (WB)
(Western Blot (WB) analysis of Tubulin beta pAb at 1:5000/1:10000/1:20000/1:40000 dilutionLane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug))
Rabbit Tubulin beta Polyclonal Antibody
Tubulin beta Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Tubulin beta; Polyclonal Antibody; Tubulin beta Polyclonal Antibody; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4; anti-Tubulin beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Tubulin beta 1/2/3/5/6/8 polyclonal antibody pAb detects endogenous levels of Tubulin beta 1/2/3/5/6/8 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-Tubulin beta antibody
Western Blot (WB)
Application Notes
WB: 1:5000-1:20,000
Immunogen
Recombinant full length Human Tubulin beta.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Tubulin beta pAb at 1:5000/1:10000/1:20000/1:40000 dilutionLane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Tubulin beta pAb at 1:5000/1:10000/1:20000/1:40000 dilutionLane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Rat (40ug) Lane7:The Brain tissue lysate of Mouse (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Tubulin beta pAb at 1:5000 dilutionLane1:A549 whole cell lysate (20ug) Lane2:PC12 whole cell lysate (20ug) Lane3:BV2 whole cell lysate (20ug) Lane4:The Kidney tissue lysate of Mouse (20ug) Lane5:The Prostate tissue lysate of Rat (20ug))
Western Blot (WB)
(Western Blot (WB) analysis of Tubulin beta pAb at 1:5000 dilutionLane1:A549 whole cell lysate (20ug) Lane2:PC12 whole cell lysate (20ug) Lane3:BV2 whole cell lysate (20ug) Lane4:The Kidney tissue lysate of Mouse (20ug) Lane5:The Prostate tissue lysate of Rat (20ug))
Related Product Information for anti-Tubulin beta antibody
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55, 000 kDa.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Similar Products
Product Notes
The Tubulin beta (Catalog #AAA3000035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulin beta Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:5000-1:20,000. Researchers should empirically determine the suitability of the Tubulin beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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