Western Blot (WB)
(Western blot analysis of lysates from PC-3, LNCaP, Hela cell line (from left to right), using Tuberin (TSC2) Antibody. MBS9211633 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human Tuberin (TSC2) Polyclonal Antibody | anti-TSC2 antibody
Tuberin (TSC2) Antibody (S1798)
Western Blot (WB)
(Western blot analysis of lysates from PC-3, LNCaP, Hela cell line (from left to right), using Tuberin (TSC2) Antibody. MBS9211633 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from PC-3, LNCaP, Hela cell line (from left to right), using Tuberin (TSC2) Antibody. MBS9211633 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, Hela, cell line (from left to right), using Tuberin (TSC2) Antibody. MBS9211633 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, Hela, cell line (from left to right), using Tuberin (TSC2) Antibody. MBS9211633 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with Tuberin (TSC2) Antibody (S1798). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with Tuberin (TSC2) Antibody (S1798). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.)
Western Blot (WB)
(Western blot analysis of TSC2-pS1798 in Ramos cell line lysates (35ug/lane). TSC2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of TSC2-pS1798 in Ramos cell line lysates (35ug/lane). TSC2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004).
Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004).
Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004).
Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).
NCBI and Uniprot Product Information
NCBI Description
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; Tumor suppressor; GAPs; Cell cycle regulation; GAPs, misc.
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: TSC1-TSC2 complex; Golgi apparatus; growth cone; cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; dendrite; caveola; nucleus; cytosol
Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding
Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; negative regulation of Wnt receptor signaling pathway; regulation of insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of cell cycle; protein heterooligomerization; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; cell projection organization and biogenesis; positive chemotaxis; protein import into nucleus; regulation of endocytosis; acute-phase response; protein kinase B signaling cascade; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; insulin receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade
Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2
Research Articles on TSC2
Similar Products
Product Notes
The TSC2 tsc2 (Catalog #AAA9211633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1776-1805. The Tuberin (TSC2) Antibody (S1798) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tuberin (TSC2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the TSC2 tsc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tuberin (TSC2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
