Rabbit anti-C elegans Tubby Polyclonal Antibody | anti-TUB antibody

Tubby (TUB, F10B5.4, Retinal Degeneration 5, RD5)

Cat. Num. AAA622373

• Gene Names: TUB; rd5
• Reactivity: C elegans
• Applications: Western Blot, Immunoprecipitation
• Purity: Serum; Whole antisera
• Synonyms: Tubby; Polyclonal Antibody; Tubby (TUB; F10B5.4; Retinal Degeneration 5; RD5); Anti -Tubby (TUB; anti-TUB antibody

• Host: Rabbit
• Reactivity: C elegans
• Clonality: Polyclonal
• Specificity: Detect C. elegans Tub-1 protein.
• Purity/Purification: Serum; Whole antisera

• Applicable Applications for anti-TUB antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000. The molecular weight of this protein as seen in Western blot is 48-50kD.
• Immunogen: A recombinant Tubby protein (domain residues 160-421).
• Cellular Localization: Cytoplasmic
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TUB antibody

Tub-1 protein is C. elegans tubby homolog, required for normal sensory behavior. It is expressed in ciliated neurons and undergoes both cilated and dendritic transport. It has been shown to be involved in regulating fat storage, most likely by modulating transport, sensing, or responding to signals in ciliated neurons. It has also been shown to play a role in regulating life span, but by a different mechanism than fat storage regulation.

Product Categories/Family for anti-TUB antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 29826279
• NCBI GeneID: 7275
• NCBI Accession #: NP_813977.1
• NCBI GenBank Nucleotide #: NM_177972.2
• UniProt Accession #: P50607; O00293; Q6B007; D3DQU4; Q96JS0; Q9H4D2
• Molecular Weight: 55,651 Da
• NCBI Official Full Name: tubby protein homolog isoform b
• NCBI Official Synonym Full Names: tubby homolog (mouse)
• NCBI Official Symbol: TUB
• NCBI Official Synonym Symbols: rd5
• NCBI Protein Information: tubby protein homolog; tubby homologue; OTTHUMP00000230766; OTTHUMP00000230767; OTTHUMP00000230768
• UniProt Protein Name: Tubby protein homolog
• Protein Family: Tubby protein
• UniProt Gene Name: TUB
• UniProt Entry Name: TUB_HUMAN

NCBI Description

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

tubby: an effector protein for heterotrimeric G protein-coupled receptors. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol-4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation by the G-alpha(q)/(11) subclass of G proteins that are linked to receptors including the seratonin receptor 5-HT(2C). Can bind DNA in vitro and may contribute to the regulation of transcription in the nucleus. TUB can be phosphorylated by the insulin receptor and may participate in insulin receptor signaling. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway. Highly expressed in the paraventricular nucleus of the hypothalamus and several other brain regions. May play a role in the hypothalamic regulation of body weight. Contributes to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. Belongs to the TUB family. Mutations in the tubby promote maturity-onset obesity in mice. Interacts with GNAQ. Interacts with TULP1. Two isoforms of the human protein are produced through alternative splicing.

Protein type: Lipid-binding; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: protein complex binding; G-protein coupled photoreceptor activity

Biological Process: sensory perception of sound; retina development in camera-type eye; positive regulation of phagocytosis; response to hormone stimulus; phototransduction; multicellular organismal macromolecule metabolic process; detection of visible light; phagocytosis

Disease: Retinal Dystrophy And Obesity