Rabbit anti-Mouse Tubby Polyclonal Antibody | anti-TUB antibody

Tubby (TULP, Tubby-like Protein, Tub Homolog)

Cat. Num. AAA610116

• Gene Names: TUB; rd5
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Tubby; Polyclonal Antibody; Tubby (TULP; Tubby-like Protein; Tub Homolog); Anti -Tubby (TULP; anti-TUB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Tubby/Tub homolog. The immunogen shows no significant sequence homology with Agouti, AGRP, TULP1 or TULP2. Species sequence homology: 95% with human Tubby and human Tub homolog (1-2).
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.4, containing 0.2% BSA and 0.05% sodium azide. Reconstitute with 100ul sterile, ddH2O or sterile 40-50% glycerol.

• Applicable Applications for anti-TUB antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:10,000-1:100,000.; Western Blot: 1-10ug/ml.
• Immunogen: A 19 amino acid peptide near the N-terminus of mouse Tubby (1).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TUB antibody

Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.

Product Categories/Family for anti-TUB antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 29826279
• NCBI GeneID: 7275
• NCBI Accession #: NP_813977.1
• NCBI GenBank Nucleotide #: NM_177972.2
• UniProt Accession #: P50607; O00293; Q6B007; D3DQU4
• Molecular Weight: 55,651 Da
• NCBI Official Full Name: tubby protein homolog isoform b
• NCBI Official Synonym Full Names: tubby bipartite transcription factor
• NCBI Official Symbol: TUB
• NCBI Official Synonym Symbols: rd5
• NCBI Protein Information: tubby protein homolog; tubby homolog; tubby homologue
• UniProt Protein Name: Tubby protein homolog
• Protein Family: Tubby protein
• UniProt Gene Name: TUB
• UniProt Entry Name: TUB_HUMAN

NCBI Description

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

tubby: an effector protein for heterotrimeric G protein-coupled receptors. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol-4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation by the G-alpha(q)/(11) subclass of G proteins that are linked to receptors including the seratonin receptor 5-HT(2C). Can bind DNA in vitro and may contribute to the regulation of transcription in the nucleus. TUB can be phosphorylated by the insulin receptor and may participate in insulin receptor signaling. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway. Highly expressed in the paraventricular nucleus of the hypothalamus and several other brain regions. May play a role in the hypothalamic regulation of body weight. Contributes to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. Belongs to the TUB family. Mutations in the tubby promote maturity-onset obesity in mice. Interacts with GNAQ. Interacts with TULP1. Two isoforms of the human protein are produced through alternative splicing.

Protein type: Membrane protein, peripheral; Lipid-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; extracellular region; plasma membrane; cytosol; nucleus

Molecular Function: protein complex binding; G-protein coupled photoreceptor activity

Biological Process: sensory perception of sound; retina development in camera-type eye; positive regulation of phagocytosis; phototransduction; response to hormone stimulus; multicellular organismal macromolecule metabolic process; detection of visible light; phagocytosis

Disease: Retinal Dystrophy And Obesity