Rabbit anti-Human, Mouse TUBB3 Polyclonal Antibody | anti-TUBB3 antibody

TUBB3, NT (TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III) (APC)

Cat. Num. AAA6360168

• Gene Names: TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TUBB3; Polyclonal Antibody; NT (TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III) (APC); Tubulin beta-III; anti-TUBB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TUBB3 at ~55kD. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TUBB3 antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa36-63 in the N-terminal region of human TUBB3, UniProt Accession #NP_006077.2; Q13509. Species sequence homology: bovine, chicken, monkey, rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of mouse brain tissue lysates (15ug/lane) usingMBS646737. This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of HepG2 cell line lysates (15ug/lane) usingMBS646737. This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human hepatocarcinoma using MBS646737 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells (right histogram) using MBS646737 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TUBB3 antibody

Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal-and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Product Categories/Family for anti-TUBB3 antibody

Antibodies; Tubulin

References

1. Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996). 2. Vinores, S.A., et al. Exp. Eye Res. 60(4):385-400(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 50592996
• NCBI GeneID: 10381
• NCBI Accession #: NP_006077.2
• NCBI GenBank Nucleotide #: NM_006086.3
• UniProt Accession #: Q13509
• NCBI Official Full Name: tubulin beta-3 chain isoform 1
• NCBI Official Synonym Full Names: tubulin beta 3 class III
• NCBI Official Symbol: TUBB3
• NCBI Official Synonym Symbols: CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• NCBI Protein Information: tubulin beta-3 chain
• UniProt Protein Name: Tubulin beta-3 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB3
• UniProt Synonym Gene Names: TUBB4
• UniProt Entry Name: TBB3_HUMAN

NCBI Description

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Uniprot Description

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding

Biological Process: protein polymerization; axon guidance; mitosis; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1