Rabbit anti-Human TSPY1 Polyclonal Antibody | anti-TSPY1 antibody

TSPY1, CT (TSPY1, TSPY, Testis-specific Y-encoded protein 1, Cancer/testis antigen 78)

Cat. Num. AAA646151

• Gene Names: TSPY1; CT78; TSPY; DYS14; pJA923
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TSPY1; Polyclonal Antibody; CT (TSPY1; TSPY; Testis-specific Y-encoded protein 1; Cancer/testis antigen 78); Anti -TSPY1; anti-TSPY1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TSPY1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: TSPY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-295 amino acids from the C-terminal region of human TSPY1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TSPY1 antibody

TSPY1 may be involved in sperm differentiation and proliferation.

Product Categories/Family for anti-TSPY1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 308522758
• NCBI GeneID: 7258
• NCBI Accession #: NP_001184171.1
• NCBI GenBank Nucleotide #: NM_001197242.1
• UniProt Accession #: Q01534; O00216; P09002; Q0VAD3; Q9UNN7; A6NJD2
• Molecular Weight: 35,012 Da
• NCBI Official Full Name: testis-specific Y-encoded protein 1 isoform TSPY-S
• NCBI Official Synonym Full Names: testis specific protein, Y-linked 1
• NCBI Official Symbol: TSPY1
• NCBI Official Synonym Symbols: CT78; TSPY; DYS14; pJA923
• NCBI Protein Information: testis-specific Y-encoded protein 1; cancer/testis antigen 78
• UniProt Protein Name: Testis-specific Y-encoded protein 1
• Protein Family: Testis-specific Y-encoded protein
• UniProt Gene Name: TSPY1
• UniProt Synonym Gene Names: TSPY; CT78
• UniProt Entry Name: TSPY1_HUMAN

NCBI Description

The protein encoded by this gene is found only in testicular tissue and may be involved in spermatogenesis. Approximately 35 copies of this gene are present in humans, but only a single, nonfunctional orthologous gene is found in mouse. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

Function: May be involved in sperm differentiation and proliferation. Ref.1

Subcellular location: Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Also found in nucleus. Ref.1

Tissue specificity: Specifically expressed in testicular tissues. Isoform 1 and isoform 2 are expressed in spermatogonia and spermatocytes. Found in early testicular carcinoma in situ, spermatogonial cells in testicular tissues of 46,X,Y female and in prostate cancer cell lines. Ref.1 Ref.11

Developmental stage: Detected in 22-week old testis. Ref.1

Induction: Up-regulated by androgen in a prostate cancer cell line. Ref.11

Post-translational modification: Phosphorylated. Ref.1

Polymorphism: Variants Val-Glu-Val-Val-Ala-Glu-79 Ins and Arg-195 are shown to be present in a number of TSPY1 copies of the Yp11 loci. Variant Arg-195 is shown to be present at least in one TSPY1 copy of the Yq locus.Maps to a tandemly repeated region on chromosome Yp11; additionally at least one copy is reported originating from Yq. The gene is thought to be present with an inter-individual variation in copy number and between 20 and 60 copies per Y chromosome are expected. Ref.3 reports 35 tandemly repeated gene copies on Yp11 originating from one individual.

Involvement in disease: TSPY is located in the gonadoblastoma critical region and is preferentially expressed in tumor germ cells of gonadoblastoma specimens. Expression also correlates with testicular seminoma and tumorigenesis of the prostate gland. Ref.2 Ref.12 Ref.13

Sequence similarities: Belongs to the nucleosome assembly protein (NAP) family.

Sequence caution: The sequence AAA36570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA61238.1 differs from that shown. Reason: Probable unspliced transcript.The sequence CAA29640.1 differs from that shown. Reason: Sequence of unknown origin inserted in the coding sequence.The sequence X74029 differs from that shown. Reason: Frameshift at several positions.