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Western Blot (WB) (Western blot analysis of extracts from 293 cells using TSPAN8 antibody.)

Rabbit anti-Human TSPAN8 Polyclonal Antibody | anti-TSPAN8 antibody

TSPAN8 Antibody

Gene Names
TSPAN8; CO-029; TM4SF3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TSPAN8; Polyclonal Antibody; TSPAN8 Antibody; anti TSPAN8; CO029; Tetraspanin 8; Tetraspanin-8; TM4SF3; Transmembrane 4 superfamily member 3; TSN8_HUMAN; Tspan 8; Tspan-8; TSPAN8 antibody; Tumor associated antigen CO 029; Tumor-associated antigen CO-029; anti-TSPAN8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TSPAN8 antibody detects endogenous levels of total TSPAN8
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
237
Applicable Applications for anti-TSPAN8 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
Gastric, colon, rectal, and pancreatic carcinomas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells using TSPAN8 antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells using TSPAN8 antibody.)

Immunofluorescene (IF)

(MBS9604574 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9604574 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-TSPAN8 antibody
Similarity: Belongs to the tetraspanin (TM4SF) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 26 kD
Predicted: 27 kDa
NCBI Official Full Name
tetraspanin-8
NCBI Official Synonym Full Names
tetraspanin 8
NCBI Official Symbol
TSPAN8
NCBI Official Synonym Symbols
CO-029; TM4SF3
NCBI Protein Information
tetraspanin-8
UniProt Protein Name
Tetraspanin-8
UniProt Gene Name
TSPAN8
UniProt Synonym Gene Names
TM4SF3; Tspan-8

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Research Articles on TSPAN8

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Product Notes

The TSPAN8 tspan8 (Catalog #AAA9604574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSPAN8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSPAN8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TSPAN8 tspan8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSPAN8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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